Antimicrobial Peptide-Conjugated Hierarchical Antifouling Polymer Brushes for Functionalized Catheter Surfaces
Antimicrobial Peptide-Conjugated Hierarchical Antifouling Polymer Brushes for Functionalized Catheter Surfaces
复制标题
用于功能化导管表面的抗菌肽共轭分层防污聚合物刷
DOI：
10.1021/acs.biomac.9b01060
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发表时间：
2019
影响因子：
6.2
通讯作者：
Fu-Jian Xu
中科院分区：
文献类型：
--
作者：
Xin-Yang Zhang;Yu-Qing Zhao;Yidan Zhang;Anzhi Wang;Xiaokang Ding;Yang Li;Shun Duan;Xuejia Ding;Fu-Jian Xu
导管相关性感染是现代医学面临的一大挑战，造成了巨大的经济负担。增加了病人的发病率。因此，对具有固有抗菌功能的表面有很大的需求。防止细菌定植和血细胞附着的特性和生物相容性。在此，我们开发了一种通过表面引发原子转移在聚氨酯（PU）上构建具有分层结构的聚合物刷的策略。自由基聚合（SI-ATRP）。制备了表面功能化聚氨酯（PU- dmh）。聚（3-[二甲基-[2-（2-甲基丙烷-2-烯氧基）乙基]氮杂酰基]丙烷-1-磺酸盐）（PDMAPS）刷为下层。抗菌肽偶联聚甲基丙烯酸（PMAA）刷为上层。PU-DMH表面。对革兰氏阳性菌和革兰氏阴性菌均表现出优良的杀菌性能，并能防止积累。表面的细菌碎片。同时，PU-DMH样品具有良好的血液相容性和低的细胞毒性。此外，还研究了PU-DMH在水动力条件下的综合防污杀菌性能。经体外循环模型证实。功能化表面具有持久的防污杀菌作用。静态和水动力条件下的性能。动物的微生物学和组织学结果。实验还验证了其体内抗感染性能。本研究在临床应用方面具有一定的前景。预防导管相关性感染。
Catheter-related infection is a great challenge to modern medicine, which causes significant economic burden.and increases patient morbidity. Hence, there is a great requirement for functionalized surfaces with inherently antibacterial.properties and biocompatibility that prevent bacterial colonization and attachment of blood cells. Herein, we developed a.strategy for constructing polymer brushes with hierarchical architecture on polyurethane (PU) via surface-initiated atomtransfer.radical polymerization (SI-ATRP). Surface-functionalized PU (PU-DMH) was readily prepared, which comprised of.poly(3-[dimethyl-[2-(2-methylprop-2-enoyloxy)ethyl]azaniumyl]propane-1-sulfonate) (PDMAPS) brushes as the lower layer.and antimicrobial peptide-conjugated poly(methacrylic acid) (PMAA) brushes as the upper layer. The PU-DMH surface.showed excellent bactericidal property against both Gram-positive and Gram-negative bacteria and could prevent accumulation.of bacterial debris on surfaces. Simultaneously, the PU-DMH samples possessed good hemocompatibility and low cytotoxicity..Furthermore, the integrated antifouling and bactericidal properties of PU-DMH under hydrodynamic conditions were.confirmed by an in vitro circulating model. The functionalized surface possessed persistent antifouling and bactericidal.performances both under static and hydrodynamic conditions. The microbiological and histological results of animal.experiments also verified the in vivo anti-infection performance. The present work might find promising clinical applications for.preventing catheter-related infection.