Expressions of cytokines and chemokines in the dorsal motor nucleus of the vagus nerve after right vagotomy
Expressions of cytokines and chemokines in the dorsal motor nucleus of the vagus nerve after right vagotomy
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DOI：
10.1016/j.molbrainres.2005.09.017
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发表时间：
2005-12-07
期刊：
影响因子：
--
通讯作者：
Tay, SSW
中科院分区：
文献类型：
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作者：
Ji, JF;Dheen, ST;Tay, SSW
本研究的目的是调查细胞因子、肿瘤坏死因子 α (TNF-α)、白细胞介素 1 β (IL-1 β)、白介素 6 (IL-6) 和转化生长因子 β 1 (TGF-1) 以及趋化因子、fratalkine、单核细胞趋化蛋白的表达。右侧迷走神经切断术后迷走神经背侧运动核中的 I (MCP-1) 和基质细胞衍生因子 I (SDF-1)。结果显示，DMV 中 IL-1β、IL-6、TGF-1、fractalkine 和 MCP-1 的免疫反应性在第 14 天时上调，并且这种上调至少持续到右侧迷走神经切断术后 28 天。定量分析显示，在右侧迷走神经切断术后 14 天和 28 天，右侧 DMV 中免疫阳性细胞的数量显着增加。此外，在右迷走神经切断术后第7天和第14天，在受伤的DMV中观察到TNF-α免疫反应性的上调和TNF-α免疫阳性细胞数量的显着增加，以及在第14天SDF-1免疫阳性细胞的增加。实时RT-PCR分析显示，迷走神经切断术后第7天IL-1β、fractalkine和MCP-1 mRNA表达显着增加，TNF-α mRNA表达在第1天上调。然而，在第1天观察到TGF-β1 mRNA表达的峰值增加，并且显着增加至少持续到右侧迷走神经切断术后14天。双免疫荧光分析显示，在右侧迷走神经切断术后 14 天，凝集素（一种小胶质细胞标记物）与 CX(3)CR1 共定位，但不与 IL-1 β 共定位。这项研究表明，参与神经保护和神经破坏的细胞因子和趋化因子可以在轴突 DMV 中被激活。然而，需要进一步研究以了解决定迷走神经切断术后迷走神经运动神经元命运的神经破坏和神经保护机制。 (c) 2005 Elsevier B.V. 保留所有权利。
The aim of this study was to investigate the expression of cytokines, tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interieukin-6 (IL-6) and transforming growth factor-beta 1 (TGF-1) and chemokines, fractalkine, monocyte chemoattractant protein. I (MCP-1) and stromal cell-derived factor I (SDF-1) in the dorsal motor nucleus of the vagus nerve (DMV) after right vagotomy. Results showed that the immunoreactivities of IL-1 beta, IL-6, TGF-1, fractalkine and MCP-1 were upregulated in the DMV at 14 days and the upregulation persisted at least until 28 days following right vagotomy. Quantification analysis revealed significant increases in the number of their immunopositive cells in the right DMV at 14 and 28 days after right vagotomy. Moreover, the upregulation of TNF-alpha immunoreactivity and significantly increased number of TNF-alpha-immunopositive cells were observed in the injured DMV at 7 and 14 days, and the increase in SDF-1-immunopositive cells at 14 days, after right vagotomy. Real time RT-PCR analysis showed the significant increase in the mRNA expression of IL-1 beta, fractalkine and MCP-1 at 7 days, and the upregulation of TNF-a mRNA expression at I day after vagotomy. However, the peak increase in TGF-beta 1 mRNA expression was observed at I day and the significant increase persisted at least until 14 days following right vagotomy. Double immunofluorescence analysis showed co-localization of lectin, a marker for microglia with CX(3)CR1 but not with IL-1 beta at 14 days following right vagotomy. This study suggests that cytokines and chemokines involved in neuroprotection and neurodestruction could be activated in the axotomized DMV. However, it warrants further investigation to understand the neurodestructive and neuroprotective mechanisms that determine the fate of the vagal motoneurons after vagotomy. (c) 2005 Elsevier B.V. All rights reserved.