Genome-Wide Analysis of Mitogen-Activated Protein Kinase Gene Family in Maize
Genome-Wide Analysis of Mitogen-Activated Protein Kinase Gene Family in Maize
复制标题
玉米丝裂原激活蛋白激酶基因家族的全基因组分析
DOI：
10.1007/s11105-013-0623-y
复制
发表时间：
2013-12-01
影响因子：
2.1
通讯作者：
Li, Dequan
中科院分区：
文献类型：
--
作者：
Liu, Yukun;Zhang, Dan;Li, Dequan
丝裂原活化蛋白激酶（MAPK）级联是植物中普遍存在的信号转导模块。MAPK作为MAPK级联反应（MAPKKK-MAPKK-MAPK）的最后一个环节，在连接上游激酶和下游底物中发挥重要作用。MAPK蛋白属于植物中的复杂基因家族，在拟南芥基因组中有20个MAPK基因，在水稻基因组中有17个，在白杨基因组中有21个。虽然玉米基因组测序已经完成，但迄今为止还没有关于玉米MAPK基因家族的全面研究报道。在本研究中，我们在玉米中鉴定了19个MAPK基因。这些ZmMPK基因属于在其他植物中发现的四个组（A-D）。分析了ZmMPK基因的遗传学、染色体定位、基因结构和功能相关性。此外，我们还讨论了MAPK基因在玉米中的进化分歧。此外，我们使用公开的微阵列数据分析了ZmMPKs的表达谱，并在玉米幼苗和成年植株中进行了表达分析。本研究的结果有助于更好地理解植物MAPK的复杂性，并为进一步研究玉米MAPK基因的功能提供参考。
Mitogen-activated protein kinase (MAPK) cascades are universal signal transduction modules in plants. As the last component of the MAPK cascade (MAPKKK-MAPKK-MAPK), MAPK plays important roles in linking upstream kinases and downstream substrates. The MAPK proteins belong to a complex gene family in plants, with 20 MAPK genes in the Arabidopsis genome, 17 in the rice genome, and 21 in the poplar genome. Although the maize genome sequencing has been completed, no comprehensive study has been reported thus far for the MAPK gene family in maize. In this study, we identified 19 MAPK genes in maize. These ZmMPK genes belong to four groups (A-D) found in other plants. The phylogeny, chromosomal location, gene structure, and the functional relevancy of ZmMPK genes were analyzed. Moreover, we discuss the evolutionary divergence of MAPK genes in maize. Furthermore, we analyzed the expression profiles of ZmMPKs using the public microarray data and performed expression analyses in maize seedlings and adult plants. The data obtained from our study contribute to a better understanding of the complexity of MAPKs in plants and provide a useful reference for further functional analysis of MAPK genes in maize.