Immunofluorescence assessment of the timing of appearance and cellular distribution of Na/K-ATPase during mouse embryogenesis.
Immunofluorescence assessment of the timing of appearance and cellular distribution of Na/K-ATPase during mouse embryogenesis.
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小鼠胚胎发生过程中 Na/K-ATP 酶出现时间和细胞分布的免疫荧光评估。
DOI：
10.1016/0012-1606(88)90241-2
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发表时间：
1988
影响因子：
2.7
通讯作者：
G. Kidder
中科院分区：
文献类型：
--
作者：
A. J. Watson;G. Kidder
我们利用大鼠肾脏Na+/K+-ATPase多克隆抗体的免疫荧光技术研究了该酶在着床前发育过程中的细胞分布和出现的时间。这种酶首先在每个卵裂球的细胞质中的桑椹胚晚期被检测到。当空化开始时，这种分布急剧变化为包围胚腔的环，限制在基底外侧细胞边缘。利用这种酶作为空化的标志，我们检测了它在用小麦胚凝集素(WGA)处理的胚胎中的表达，WGA会导致卵裂停止，据报道在早期卵裂阶段会触发过早压实和空化样事件(L.V.Johnson 1986，Dev.Biol.113，1-9)。尽管WGA处理的2-、4-和8-细胞胚胎很快经历了致密和空化样事件，但没有观察到Na+/K+-ATPase的表达。因此，WGA效应不太可能涉及空化发展程序的加速。然而，在8-细胞期停滞但培养到第4天的胚胎以典型的囊胚模式(在每个充满液体的腔周围)表达这种酶。我们的结论是，Na+/K+-ATPase的表达在桑椹胚晚期启动或显著增加，并且不依赖于胞质分裂。该酶在空化过程中的分布与其在跨跨膜外胚层液体运输中的假定作用一致。
We have employed immunofluorescence with a rat kidney Na+/K+-ATPase polyclonal antibody to investigate the cellular distribution and timing of appearance of this enzyme during preimplantation development. The enzyme is first detected in the late morula within the cytoplasm of each blastomere. When cavitation begins this distribution changes dramatically to a ring encircling the blastocoel, restricted to the basolateral cell margins. Using this enzyme as a marker for cavitation, we examined its expression in embryos that had been treated with wheat germ agglutinin (WGA), which causes cleavage arrest and was reported to trigger premature compaction- and cavitation-like events in early cleavage stages (L. V. Johnson 1986,Dev. Biol.113,1–9). Although WGA-treated 2-,4-, and 8-cell embryos quickly underwent compaction- and cavitation-like events, no Na+/K+-ATPase expression was observed. Thus the WGA effect does not likely involve acceleration of the developmental program for cavitation. Embryos arrested at the 8-cell stage but cultured overnight to Day 4, however, expressed the enzyme in the typical blastocyst pattern (around each fluid-filled cavity). We conclude that Na+/K+-ATPase expression is initiated or increases dramatically in the late morula and is independent of cytokinesis. The enzyme assumes a distribution during cavitation consistent with its presumed role in transtrophectodermal fluid transport.