Separation of corticosteroids and aromatic hydrocarbons by cyclodextrin modified micellar electrokinetic chromatography
Separation of corticosteroids and aromatic hydrocarbons by cyclodextrin modified micellar electrokinetic chromatography
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环糊精修饰胶束电动色谱法分离皮质类固醇和芳香烃
DOI：
10.1080/01483919108049298
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发表时间：
1991
影响因子：
1.3
通讯作者：
M. Matsuo
中科院分区：
文献类型：
--
作者：
H. Nishi;M. Matsuo
摘要胶束电动色谱（MEKC）可以通过电泳技术分离不带电或电中性的化合物，但高度亲脂的化合物，如，皮质类固醇和芳烃不能通过MEKC与十二烷基硫酸钠（SDS）溶液分离，因为这些溶质由于其大的胶束增溶作用而以与SDS胶束几乎相同的速度迁移。在SDS溶液中加入环糊精（CD），即环糊精修饰的胶束电动色谱（CD/MEKC），显著提高了这些亲脂性化合物的分离度。考察了不同浓度和不同类型的环糊精对皮质类固醇和芳烃分离的影响。
Abstract Micellar electrokinetic chromatography (MEKC) permits the separation of uncharged or electrically neutral compounds by the electrophoretic technique, but highly lipophilic compounds, e.g., corticosteroids and aromatic hydrocarbons could not be resolved by MEKC with sodium dodecyl sulfate (SDS) solutions because such solutes migrated with almost the same velocity as that of the SDS micelle, owing to their large micellar solubilization. the addition of cyclodextrins (CDs) to the SDS solution, that is, cyclodextrin-modified micellar electrokinetic chromatography (CD/MEKC), remarkably improved the resolution of these lipophilic compounds. the effects of concentration and the type of CDs on the separation of corticosteroids and aromatic hydrocarbons were investigated.