Kinetics of glutamate and γ-aminobutyric acid in cerebrospinal fluid in a canine model of complex partial status epilepticus induced by kainic acid
Kinetics of glutamate and γ-aminobutyric acid in cerebrospinal fluid in a canine model of complex partial status epilepticus induced by kainic acid
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DOI：
10.1292/jvms.66.1555
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发表时间：
2004-12-01
影响因子：
1.2
通讯作者：
Orima, H
中科院分区：
文献类型：
--
作者：
Hasegawa, D;Matsuki, N;Orima, H
大脑中兴奋性和抑制性递质的不平衡被认为是导致癫痫发作的原因。在这项研究中，我们研究了谷氨酸（GLU）和γ-氨基丁酸（GABA）在脑脊液（CSF-GLU和CSF-GABA，分别）的动力学在复杂的部分癫痫持续状态（CPSE）的犬模型，通过微量注射红藻氨酸（KA）到一侧杏仁核。在急性期（CPSE发作后3、6、12和48小时），CSF-GLU显著升高，而CSF-GABA降低，但不显著。在慢性期，CSF-GLU和CSF-GABA在CPSE发作后72小时显著低于正常，2个月时恢复正常。本研究的结果表明，CSF-GLU是逐渐增加与癫痫发作的严重程度，并建议的可能性，CSF-GABA是一致的减少，在CPSE由KA在狗诱导。
An imbalance of excitatory and inhibitory transmitters in the brain has been suggested to cause epileptic seizures. In this study, we investigated the kinetics of glutamate (GLU) and gamma-aminobutyric acid (GABA) in cerebrospinal fluid (CSF-GLU and CSF-GABA, respectively) using a high performance liquid chromatography (HPLC) in a canine model of complex partial status epilepticus (CPSE) induced by the microinjection of kainic acid (KA) into the unilateral amygdala. During the acute phase (3, 6, 12 and 48 hr after the onset of CPSE), CSF-GLU was significantly increased, while CSF-GABA was decreased, although not significantly. In the chronic phase, both CSF-GLU and CSF-GABA were significantly lower than normal at 72 hr after the onset of CPSE, and their levels returned to normal at 2 months. Results of the present study demonstrate that CSF-GLU is gradually increased in relation with seizure severity, and suggested the possibility that CSF-GABA was consistently decreased during CPSE induced by KA in dogs.