MOLECULAR CHARACTERIZATION AND HISTOPATHOLOGY OF MYXOBOLUS KOI INFECTING THE GILLS OF A KOI, CYPRINUS CARPIO, WITH AN AMENDED MORPHOLOGICAL DESCRIPTION OF THE AGENT
MOLECULAR CHARACTERIZATION AND HISTOPATHOLOGY OF MYXOBOLUS KOI INFECTING THE GILLS OF A KOI, CYPRINUS CARPIO, WITH AN AMENDED MORPHOLOGICAL DESCRIPTION OF THE AGENT
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DOI：
10.1645/ge-2113.1
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发表时间：
2010-02-01
影响因子：
1.3
通讯作者：
Griffin, Matt J.
中科院分区：
文献类型：
--
作者：
Camus, Alvin C.;Griffin, Matt J.
一种粘虫属植物。形态上类似于富山稻。从锦鲤的鳃中分离出了longisporus和M. koi。死在观赏池塘里的鲤鱼。大的疟原虫局限于片层内，严重破坏了正常的鳃结构，足以影响呼吸。虽然病例分离物与上述物种有几个共同特征，但几个关键特征与锦鲤最相容。孢子长15.4 (14.5 ~ 16.5)μ m，宽8.3 (7.1 ~ 9.0)μ m。极性蒴果呈梨形和细长状，长10.1 (9.0-10.9)μ m，宽3.1 (2.5-3.5)μ m。极丝垂直于孢子长轴缠绕10圈（9-11）。使用生成的18S SSU rDNA序列进行BLAST搜索没有发现直接匹配。系统发育分析表明，该序列与感染鲤科鱼类鳃的Myxobolus属分支C. carpio的M. longisporus和M. toyamai亲缘关系密切（最大简约性和最小进化距离分析的自支持率为89%或更高）。尽管已发表的描述不一致，但与该分离物的形态测量相似性表明了锦鲤的形态型。本文补充了锦鲤的致病性和孢子形态的新数据，并利用iss小亚基rDNA序列数据在分子水平上进行了表征。
A Myxobolus sp.. morphologically resembling M. toyamai. longisporus, and M. koi, was isolated from the gills of a koi. Cyprinus carpio), that died in an ornamental pond. Large plasmodia were localized within lamellae, causing severe disruption of the normal branchial architecture, sufficient to compromise respiration. Although the case isolate shared several features with the aforementioned species, several key characteristics were most compatible with M. koi. In valvular view, spores were elongate and pyriform with a rounded posterior, 15.4 (14.5-16.5) mu m long and 8.3 (7.1-9.0) mu m wide. Polar capsules were pyriform and elongate, 10.1 (9.0-10.9) mu m long and 3.1 (2.5-3.5) mu m wide. Polar filaments were coiled perpendicular to the long axis of the spore making 10 turns (9-11). A BLAST search using a generated 18S SSU rDNA sequence resulted in no direct matches. Phylogenetic analysis of the sequence indicates a close relationship to M. longisporus and M. toyamai from C. carpio, within the clade of Myxobolus species infecting gills of cyprinid fishes (89% or greater bootstrap support by maximum parsimony and minimum evolution distance analysis). Although published descriptions are inconsistent, morphometric similarities to this isolate suggest a morphotype of M. koi. The description of M. koi is supplemented here with new data on pathogenicity and spore morphology and is characterized at the molecular level using I SS small subunit rDNA sequence data.