Porcine T-helper and regulatory T cells exhibit versatile mRNA expression capabilities for cytokines and co-stimulatory molecules
Porcine T-helper and regulatory T cells exhibit versatile mRNA expression capabilities for cytokines and co-stimulatory molecules
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DOI：
10.1016/j.cyto.2012.07.007
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发表时间：
2012-11-01
期刊：
影响因子：
3.8
通讯作者：
Duvigneau, J. Catharina
中科院分区：
文献类型：
--
作者：
Kaeser, Tobias;Muellebner, Andrea;Duvigneau, J. Catharina
辅助性T细胞（TH）和调节性T细胞（Tcells）是免疫应答的重要调节剂。本研究的目的是分析它们对细胞因子和其他免疫相关分子的表达潜力。因此，通过RT-qPCR分析了猪PBMC、CD 4 ㈠细胞、CD 4（+）CD 25 ㈠静息、CD 4（+）CD 25（dim）活化的TH细胞和CD 4（+）CD 25（高）Th细胞离体或在用CD 3和IL-2体外刺激后的mRNA表达潜力。在PBMC、CD 4（-）和CD 4（+）细胞中，IL-6、TNF α和TH（IL-2、IL-4、IL-17、IFN-γ）细胞因子以及免疫抑制细胞因子（IL-10和TGF-β）的多样性产生。静止和活化的TH细胞表现出各种免疫调节分子的表达增加，表明猪TH细胞具有不同的免疫技能，以应对实际的免疫情况。相比之下，Tglutamine似乎主要实现免疫抑制功能，其特征在于IL-10、IL-35、CD 40 L和CD 25的产生增加。(C)2012爱思唯尔有限公司保留所有权利。
T-helper (TH) and regulatory T cells (Tregs) are important modulators of immune responses. Aim of this study was to analyse their expression potential for cytokines and other immune-relevant molecules. Therefore, porcine PBMC, CD4(-) cells, CD4(+)CD25(-) resting, CD4(+)CD25(dim) activated TH cells, and CD4(+)CD25(high) Tregs were analysed on their mRNA expression potential ex vivo or after in vitro stimulation with CD3 and IL-2 by RT-qPCR.In vitro stimulation led to an increased production of pro-inflammatory (IL-6, TNF alpha) and TH (IL-2, IL-4, IL-17, IFN-gamma) cytokines and a diverse production of immunosuppressive cytokines (IL-10 and TGF-beta) in PBMC, CD4(-), and CD4(+) cells. Resting and activated TH cells showed an increased expression of various immune-modulatory molecules indicating that porcine TH cells possess distinct immunological skills in order to react on the actual immune situation. In contrast, Tregs appear to fulfil mainly immunosuppressive functions characterized by increased production of IL-10, IL-35, CD40L, and CD25. (C) 2012 Elsevier Ltd. All rights reserved.