Microbial comparison of smoker and non-smoker adult and early-onset periodontitis patients by polymerase chain reaction
Microbial comparison of smoker and non-smoker adult and early-onset periodontitis patients by polymerase chain reaction
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DOI：
10.1034/j.1600-051x.2000.027006417.x
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发表时间：
2000-06-01
影响因子：
6.7
通讯作者：
Kinane, DF
中科院分区：
文献类型：
--
作者：
Darby, IB;Hedge, PJ;Kinane, DF
牙周炎的病因学涉及许多细菌物种，包括伴放线放线杆菌、牙龈卟啉单胞菌、中间普雷沃氏菌、拟杆菌和齿垢密螺旋体。几项研究表明，不同形式的牙周病之间的微生物区系存在差异。人们认识到吸烟是牙周病的一个危险因素，但关于吸烟是否对牙周菌群有影响的报道相互矛盾。我们利用聚合酶链反应来确定是否存在A。放线菌共生菌、牙龈卟啉单胞菌、中间卟啉单胞菌、B. alphanthus和T. 33例成人牙周炎（AP）患者和24例全身早发性牙周炎（GEOP）患者治疗前龈下菌斑样本中的齿垢。当比较GEOP和AP患者时，两种A的阳性患者和部位的数量有显着差异。放线菌共生菌和B.因此，（分别为p=0.0023和0.00001）。在吸烟者和非吸烟者组之间，这些微生物的患病率没有统计学显著差异。这些结果证实了AP和GEOP站点具有不同的微生物区系，但表明B.和A.在该组GEOP中检测到的伴放线菌显著高于所研究的AP患者。我们的研究结果不支持吸烟者与非吸烟者牙周病原体患病率有显著差异的假设。
A number of bacterial species are involved in the aetiology of periodontitis and include Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus and Treponema denticola. Several studies have shown differences in the microflora between the various forms of periodontal disease. It is recognised that smoking is a risk factor for periodontal disease, but there are conflicting reports on whether or not smoking has an effect on the periodontal microflora. We utilised the polymerase chain reaction to determine the presence of A. actinomycetemcomitans, P. gingivalis, P. intermedia, B. forsythus and T. denticola in subgingival plaque samples in 33 adult periodontitis (AP) patients and 24 generalized early-onset periodontitis (GEOP) patients prior to treatment. When GEOP and AP patients were compared there were significant differences in the number of positive patients and sites for both A. actinomycetemcomitans and B. forsythus (p=0.0023 and 0.00001, respectively). No statistically significant differences in the prevalence of these organisms were found between smoker and non-smoker groups. These results confirm that AP and GEOP sites harbour varied microflora, but show that B. forsythus and A. actinomycetemcomitans were detected to a significantly greater extent in this group of GEOP than in the AP patients investigated. Our findings do not support the hypothesis that smokers have significant differences in the prevalence of periodontal pathogens from non-smokers.