Osteoblasts express the inflammatory cytokine interleukin-6 in a murine model of staphylococcus aureus osteomyelitis and infected human bone tissue
Osteoblasts express the inflammatory cytokine interleukin-6 in a murine model of staphylococcus aureus osteomyelitis and infected human bone tissue
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DOI：
10.1016/s0002-9440(10)63226-9
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发表时间：
2004-04-01
影响因子：
6
通讯作者：
Bost, KL
中科院分区：
文献类型：
--
作者：
Marriott, I;Gray, DL;Bost, KL
金黄色葡萄球菌是人类骨髓炎最常见的单一病因。S.近年来，金黄色葡萄球菌感染急剧增加，部分原因是社区获得性抗生素耐药菌株的出现。因此，了解这种生物体的发病机制已成为当务之急。最近，我们描述了骨形成成骨细胞在暴露于S.金黄色葡萄球菌。在本研究中，我们提供了第一个证据，在体内生产的成骨细胞在细菌感染的骨这样的分子。这些研究表明，在新生小鼠颅骨器官培养成骨细胞的关键炎症细胞因子白细胞介素-6的表达，并在体内使用的小鼠模型，非常类似的创伤诱导的葡萄球菌性骨霉菌炎的病理，如通过共聚焦。微观分析重要的是，我们已经在感染的人骨组织中建立了这些发现的临床相关性。金黄色相关性骨髓炎。因此，这些研究表明，在骨疾病如骨髓炎期间成骨细胞的细菌挑战诱导细胞产生炎性分子，其可以指导适当的宿主反应或有助于进行性炎性损伤。
Staphylococcus aureus is the single most common cause of osteomyelitis in humans. incidences of osteomyelitis caused by S. aureus have increased dramatically in recent years, in part due to the appearance of community-acquired antibiotic resistant strains. Therefore, understanding the pathogenesis of this organism has become imperative. Recently, we have described the surprising ability of bone-forming osteoblasts to secrete a number of important immune mediators when exposed to S. aureus in vitro. In the present study, we provide the first evidence for the in vivo production of such molecules by osteoblasts during bacterial infection of bone. These studies demonstrate the expression of the key inflammatory cytokine interleukin-6 by osteoblasts in organ cultures of neonatal mouse calvaria, and in vivo using a mouse model that closely resembles the pathology of trauma-induced staphylococcal osteomyclitis, as determined by confocal. microscopic analysis. Importantly, we have established the clinical relevancy of these findings in infected human bone tissue from patients with S. aureus-associated osteomyelitis. As such, these studies demonstrate that bacterial challenge of osteoblasts during bone diseases, such as osteomyelitis, induces cells to produce inflammatory molecules that can direct appropriate host responses or contribute to progressive inflammatory damage.