Combining Transient Expression and Cryo-EM to Obtain High-Resolution Structures of Luteovirid Particles
Combining Transient Expression and Cryo-EM to Obtain High-Resolution Structures of Luteovirid Particles
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DOI：
10.1016/j.str.2019.09.010
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发表时间：
2019-12-03
期刊：
影响因子：
5.7
通讯作者：
Ranson, Neil A.
中科院分区：
文献类型：
--
作者：
Byrne, Matthew J.;Steele, John F. C.;Ranson, Neil A.
黄病毒科（Luteoviridae）是致病性植物病毒，在世界范围内造成重大作物损失。它们感染广泛的粮食作物，包括谷物、豆类、葫芦科植物、甜菜、甘蔗和马铃薯，因此是对全球粮食安全的主要威胁。病毒复制被严格限制在植物脉管系统中，并且这种韧皮部限制，再加上蚜虫传播病毒颗粒的需要，使得难以产生高分辨率结构研究所需的数量的病毒。在这里，我们利用在植物中异源表达的最新进展，以产生足够数量的病毒样颗粒的结构研究。我们已经确定了它们的结构，以高分辨率的冷冻电子显微镜，提供所需的分子水平的洞察力，合理地询问luteovirid衣壳的形成和蚜虫的传播，从而提供了一个平台，为这个重要的家庭的植物病毒的预防性农药的发展。
The Luteoviridae are pathogenic plant viruses responsible for significant crop losses worldwide. They infect a wide range of food crops, including cereals, legumes, cucurbits, sugar beet, sugarcane, and potato and, as such, are a major threat to global food security. Viral replication is strictly limited to the plant vasculature, and this phloem limitation, coupled with the need for aphid transmission of virus particles, has made it difficult to generate virus in the quantities needed for high-resolution structural studies. Here, we exploit recent advances in heterologous expression in plants to produce sufficient quantities of virus-like particles for structural studies. We have determined their structures to high resolution by cryoelectron microscopy, providing the molecular-level insight required to rationally interrogate luteovirid capsid formation and aphid transmission, thereby providing a platform for the development of preventive agrochemicals for this important family of plant viruses.