Selection of rabbit CD4- CD8- T cell receptor-gamma/delta cells by in vitro transformation with human T lymphotropic virus-I.
Selection of rabbit CD4- CD8- T cell receptor-gamma/delta cells by in vitro transformation with human T lymphotropic virus-I.
复制标题
通过人类T淋巴细胞性病毒-I的体外转化，选择兔CD4-CD4-T-T细胞受体粉 - /三角洲细胞。
DOI：
10.1084/jem.178.4.1337
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发表时间：
1993-10-01
影响因子：
15.3
通讯作者：
Kindt, T J
中科院分区：
文献类型：
--
作者：
Sawasdikosol, S;Hague, B F;Zhao, T M;Bowers, F S;Simpson, R M;Robinson, M;Kindt, T J
在体外转化兔外周血单个核细胞（PBMC）与人T淋巴细胞病毒-I（HTLV）感染的人或兔细胞产生的CD 4-CD 8-细胞系，其中一些导致急性白血病时，注入兔。对来自具有不同致病作用的细胞系的前病毒的结构分析未提供与致死率的明确相关性。兔系暂时被指定为T细胞，因为它们表达白细胞介素2 R（IL-2 R）和CD 5并且缺乏表面免疫球蛋白，但没有表达功能性T细胞受体（TCR）α或β转录物。需要对HTLV-I感染的细胞进行更详细的表征，以确定细胞谱系及其对致病后果的潜在影响。衍生兔TCR γ和δ基因的探针，并用于检测γ和δ TCR RNA转录物，将体外转化的细胞系鉴定为γ/δ T细胞。CD 4+和CD 8+细胞系来源于HTLV-I感染兔的PBMC，CD 4 + TCR-α/β HTLV-I细胞系来源于兔胸腺，排除了此处使用的HTLV-I分离物仅转化CD 4-CD 8-TCR-γ/δ细胞的可能性。兔PBMC中γ/δ细胞的百分比相对较高（成年兔为23%）;这与补充IL-2的PBMC或胸腺细胞培养物中CD 4+和CD 8+细胞的减少可能解释了体外选择兔HTLV-I感染的γ/δ T细胞系的原因。在兔HTLV-I疾病模型中具有不同体内效应的良好表征的T细胞系的可用性允许评价细胞类型在HTLV-I介导的疾病中所起的作用。
In vitro transformation of rabbit peripheral blood mononuclear cells (PBMC) with human T lymphotropic virus-I (HTLV)-infected human or rabbit cells resulted in CD4- CD8- cell lines, some of which caused acute leukemia when injected into rabbits. Structural analyses of the proviruses from cell lines with diverse pathogenic effects provided no clear correlation with lethality. The rabbit lines were provisionally designated T cells because they express interleukin 2R (IL-2R) and CD5 and lack surface immunoglobulin, but none express functional T cell receptor (TCR) alpha or beta transcripts. A more detailed characterization of the HTLV-I-infected cells was required to determine cell lineage and its potential influence on pathogenic consequences. Probes for rabbit TCR gamma and delta genes were derived and used to detect gamma and delta TCR RNA transcripts, identifying the in vitro transformed lines as gamma/delta T cells. CD4+ and CD8+ lines were derived from PBMC of HTLV-I-infected rabbits and CD4+ TCR-alpha/beta HTLV-I lines were derived from rabbit thymus, eliminating the possibility that the HTLV-I isolates used here transform only CD4- CD8- TCR-gamma/delta cells. The percentage of gamma/delta cells in rabbit PBMC is relatively high (23% in adult rabbits); this with diminution of CD4+ and CD8+ cells in IL-2-supplemented PBMC or thymocyte cultures may account for selection of rabbit HTLV-I-infected gamma/delta T cell lines in vitro. The availability of well-characterized T cell lines with diverse in vivo effects in the rabbit HTLV-I disease model allows evaluation of roles played by cell type in HTLV-I-mediated disease.