Involvement of superoxide generated by NADPH oxidase in the shedding of procoagulant vesicles from human monocytic cells exposed to bupivacaine
Involvement of superoxide generated by NADPH oxidase in the shedding of procoagulant vesicles from human monocytic cells exposed to bupivacaine
复制标题
NADPH 氧化酶产生的超氧化物参与暴露于布比卡因的人单核细胞促凝血囊泡的脱落
DOI：
10.1007/s11239-017-1531-z
复制
发表时间：
2017
影响因子：
4
通讯作者：
Matsumoto Nobuyuki
中科院分区：
文献类型：
--
作者：
Azma Toshiharu;Ogawa Saori;Nishioka Akira;Kinoshita Hiroyuki;Kawahito Shinji;Nagasaka Hiroshi;Matsumoto Nobuyuki
已知表达组织因子的各种大小的促凝血囊泡通过与诱导细胞凋亡相关的机制从包括单核细胞在内的几种类型的细胞中释放，而尚未评价超氧化物是否参与此类囊泡的产生。在这里，我们报告说，局部麻醉布比卡因诱导凋亡的人单核细胞THP-1在很短的观察期内，其中脱落的促凝血囊泡相关。使用流式细胞术通过在新鲜冷冻血浆存在下FITC缀合的纤维蛋白原与囊泡的结合以及肝素对这种结合的抑制来评价作为促凝血囊泡的性质。布比卡因（1 mg/ml）增加凋亡细胞和促凝血小泡。LY 294002（100 µM）抑制NADPH氧化酶的细胞内组分的募集以构建该酶复合物的活化形式，或超氧化物歧化酶（1500单位/ml）抑制布比卡因引起的细胞凋亡诱导和促凝血囊泡的增加。我们建议，这个简单的实验系统是有用的，以探讨超氧化物的作用在人单核细胞的促凝囊泡脱落的分子机制。
It is known that a variety of sized procoagulant vesicles that express tissue factor are released from several types of cells including monocytes by mechanisms related to the induction of apoptosis, while it has not yet been evaluated whether superoxide is involved in the production of such vesicles. Here, we report that a local anesthetic bupivacaine induces apoptosis in human monocytic cells THP-1 within a short observation period, where the shedding of procoagulant vesicles is associated. The property as procoagulant vesicles was evaluated using flow cytometry by the binding of FITC-conjugated fibrinogen to vesicles in the presence of fresh frozen plasma and the suppression of this binding by heparin. Bupivacaine (1 mg/ml) increased the apoptotic cells and procoagulant vesicles. LY294002 (100 µM), that inhibits the recruiting of intracellular component of NADPH oxidase to construct the activated form of this enzyme complex, or superoxide dismutase (1500 unit/ml) suppressed bupivacaine-provoked induction of apoptosis and the increase of procoagulant vesicles. We suggest that this simple experimental system is useful to explore the molecular mechanisms of action of superoxide in the shedding of procoagulant vesicles from human monocytic cells.
登录
查看更多内容
凝块形成的粘弹性测量：血小板功能的新测试。
影响因子：
0.8
作者：
A. Saleem;C. Blifeld;S. A. Saleh;D. Yawn;M. Mace;M. Schwartz;E. Crawford
通讯作者：
E. Crawford
影响因子：
15.9
作者：
Hiraoka, W;Vazquez, N;Pommier, Y
通讯作者：
Pommier, Y
流式细胞术分析人单核细胞中钙通过细胞膜流入引起的促凝血颗粒的脱落
影响因子：
--
作者：
Ito T;Azma T;Ogawa S;Kinoshita H;Kawahito S;Matsumoto N
通讯作者：
Matsumoto N
Hydroethidine：一种荧光氧化还原探针，用于定位球状体和鼠肿瘤中的缺氧细胞。
影响因子：
8.8
作者：
Olive,PL
通讯作者：
Olive,PL
影响因子：
8.3
作者：
IMAIZUMI, S;WOOLWORTH, V;CHAN, PH
通讯作者：
CHAN, PH