Measurement of differences in pO(2) in response to perfluorocarbon carbogen in FSa and NFSa murine fibrosarcomas with low-frequency electron paramagnetic resonance oximetry
Measurement of differences in pO(2) in response to perfluorocarbon carbogen in FSa and NFSa murine fibrosarcomas with low-frequency electron paramagnetic resonance oximetry
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DOI：
10.2307/3579280
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发表时间：
1996-05-01
影响因子：
3.4
通讯作者：
Teicher, BA
中科院分区：
文献类型：
--
作者：
Halpern, HJ;Yu, C;Teicher, BA
我们使用甚低频电子顺磁共振（EPR）血氧仪测量了在C3H小鼠腿内植入FSa和NFSa纤维肉瘤后，灌注全氟辛基溴（PFOB）后呼吸气氛变化引起的氧浓度（δ pO(2)）的变化。在给予高密度（47% v/v）全氟化碳pff，全氟隆（Alliance pharmaceutical Corporation, San Diego， CA）之前和之后对每个肿瘤进行测量。在给药PFOB后，在给药前（PFOB/空气）和给药后（95% O-2 + 5% CO2， PFOB/碳）对每个肿瘤进行测量。与PFOB/空气氧合相比，PFOB/碳处理的δ pO(2)变化较大。在有PFOB和没有PFOB的呼吸小鼠之间，氧浓度没有显著差异。FSa肿瘤的平均δ pO(2)为13 +/- 6 torr， NFSa纤维肉瘤的平均δ pO(2)为28 +/- 7 torr。肿瘤间差异如此之大，以至于在缺氧程度越高的FSa肿瘤中变化越小的趋势并不显著（P = 0.13）。本文介绍了一种测量肿瘤组织氧合差异的新方法。这些测量结果表明，在相似组织学的pfob输注肿瘤中，pO(2)对不同呼吸气氛的反应存在很大差异。肿瘤间δ pO(2)的差异可能与放射反应的差异有关。(C) 1996年，辐射研究学会
We have used very low-frequency electron paramagnetic resonance (EPR) oximetry to measure the change in oxygen concentration (Delta pO(2)) due to change in breathing atmosphere in FSa and NFSa fibrosarcomas implanted in the legs of C3H mice infused with perfluoro-octylbromine (PFOB). Measurements in each tumor were made before and after the administration of the high-density (47% v/v) perfluorocarbon PFOB, perflubron (Alliance pharmaceutical Corporation, San Diego, CA). Measurements in each tumor were also made, after the administration of the PFOB, both before (PFOB/air) and after the administration of carbogen (95% O-2 + 5% CO2, PFOB/carbogen). Large changes (Delta pO(2)) relative to PFOB/air oxygenation were seen with the administration of PFOB/carbogen. No significant difference in oxygen concentration was seen between air-breathing mice with and without PFOB. The mean Delta pO(2) for FSa tumors was 13 +/- 6 torr, while the mean for NFSa fibrosarcomas was 28 +/- 7 torr. There were such large intertumor differences that the trend toward a smaller change in the more hypoxic FSa tumors was not significant (P = 0.13). This paper describes a novel method of measuring differences in oxygenation in tumor tissues. The results of such measurements indicate large differences in pO(2) response to different breathing atmospheres in PFOB-infused tumors of similar histology. The intertumor Delta pO(2) differences may correlate with differences in radiation response. (C) 1996 by Radiation Research Society