Multifunctional polyvinylpyrrolidinone-polyacrylic acid copolymer hydrogels for biomedical applications
Multifunctional polyvinylpyrrolidinone-polyacrylic acid copolymer hydrogels for biomedical applications
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DOI：
10.1016/j.ijpharm.2006.07.008
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发表时间：
2006-12-01
影响因子：
5.8
通讯作者：
Higginbotham, Clement L.
中科院分区：
文献类型：
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作者：
Devine, Declan M.;Devery, Sinead M.;Higginbotham, Clement L.
用紫外光聚合法制备了N-乙烯基吡咯烷酮与丙烯酸的共聚物，并用二甲基丙烯酸乙二醇酯和二甲基丙烯酸聚乙二醇600进行交联。通过在100 ℃下对样品进行索氏浸提72 h，分析这些聚合物的可浸提物含量。阿司匹林和扑热息痛以25重量%掺入聚合物结构中。在固化过程中，它们的存在通过傅里叶变换红外光谱法证实。发现药物从聚合物基质中的释放速率取决于聚合物和活性剂之间的分子间键合，在所有情况下，阿司匹林的释放都比对乙酰氨基酚慢。结果表明，扑热息痛在24小时后完全释放，而阿司匹林的完全释放需要长达70小时。最后，对交联聚乙烯吡咯烷酮进行了初步的体外生物相容性试验，通过在MTT试验中测定人肝癌HepG 2细胞活力，并在彗星试验中测定与各种浓度的含聚乙烯吡咯烷酮的培养基直接接触后的DNA损伤。细胞毒性数据表明，两种交联剂的剂量依赖性效应，浓度范围为0.025-2.5 mg/ml（-1）时，活性略有下降，最多为未处理细胞的70%。再次，在短期暴露于EGDMA交联水凝胶后的彗星测定中的DNA迁移与MTT数据相关。(c)2006 Elsevier B. V.保留所有权利。
Copolymers of N-vinylpyrrolidinone and acrylic acid, crosslinked with ethylene glycol dimethacrylate and polyethylene glycol 600 dimethacrylate were prepared by UV-polymerisation. These polymers were analysed for their extractable content by Soxhlet extraction of the samples at 100 degrees C for 72 h. Aspirin and paracetamol were incorporated into the polymer structure at 25 wt.% during the curing process and their presence confirmed by Fourier transform infrared spectroscopy. It was found that the release rate of the drug from the polymer matrix was dependent on intermolecular bonding between the polymer and active agent with aspirin being released slower than paracetamol in all cases. Results showed that paracetamol was completely released after 24 h whereas complete release of aspirin took up to 70 h. Finally preliminary in vitro biocompatibility testing was performed for crosslinked polyvinylpyrrolidinone, by determining human hepatoma HepG2 cell viability in the MTT assay and DNA damage in the comet assay following direct contact with various concentrations of polyvinylpyrrolidinone-containing media. Cytotoxicity data suggests a dose-dependent effect for both crosslinkers, with concentrations in the range 0.025-2.5 mg ml(-1) showing a marginal decrease in viability to, at most, 70% that of untreated cells. Again DNA migration in the comet assay following short-term exposure to EGDMA crosslinked hydrogels correlates with MTT data. (c) 2006 Elsevier B.V. All rights reserved.