Tuning Molecular Adhesion via Material Anisotropy
Tuning Molecular Adhesion via Material Anisotropy
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通过材料各向异性调节分子粘附
DOI：
10.1002/adfm.201300069
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发表时间：
2013-10-04
影响因子：
19
通讯作者：
Gao, Huajian
中科院分区：
文献类型：
--
作者：
Zhang, Wenliang;Lin, Yuan;Gao, Huajian
细胞与细胞外基质的黏附依赖于顺应性细胞-基质界面上大量受体-配体键的集体行为。虽然大多数生物组织和结构，包括细胞和细胞外基质，表现出强烈的各向异性材料性质，但现有的研究通过受体-配体键的分子粘附性在很大程度上局限于各向同性材料。横观各向同性是生物系统中材料各向异性的一种常见形式，在此我们研究了横向各向同性在调节受体-配体键簇的黏附行为中的作用。这些结果为理解细胞在各向异性细胞外基质上的黏附以及探索通过材料性质的各向异性设计来控制细胞黏附的可能性提供了理论基础。结合分析和模拟表明，材料各向异性的取向强烈影响粘结剂的表观柔软性，从而使粘结剂的整体寿命改变几个数量级。这项研究的一个含义是，通过重塑细胞外基质或细胞骨架中的材料各向异性，可以实现不同的细胞行为。不同加载条件的比较，以及材料各向异性的影响，在含反应物的软表面之间的分子相互作用中产生了丰富的非平衡行为，对细胞的机械敏感性具有重要的意义。
Cell adhesion with extracellular matrix depends on the collective behaviors of a large number of receptor-ligand bonds at the compliant cell-matrix interface. While most biological tissues and structures, including cells and extracellular matrices, exhibit strongly anisotropic material properties, existing studies on molecular adhesion via receptor-ligand bonds have been largely limited to isotropic materials. Here the effects of transverse isotropy, a common form of material anisotropy in biological systems, in modulating the adhesion behavior of a cluster of receptor-ligand bonds are investigated. The results provide a theoretical basis to understand cell adhesion on anisotropic extracellular matrices and to explore the possibility of controlling cell adhesion via anisotropy design in material properties. The combined analysis and simulations show that the orientation of material anisotropy strongly affects the apparent softness felt by the adhesive bonds, thereby altering their ensemble lifetime by several orders of magnitude. An implication of this study is that distinct cellular behaviors can be achieved through remodeling of material anisotropy in either extracellular matrix or cytoskeleton. Comparison between different loading conditions, together with the effects of material anisotropy, yields a rich array of out-of-equilibrium behaviors in the molecular interaction between reactant-bearing soft surfaces, with important implications on the mechanosensitivity of cells.