UNIQUE RNA SPECIES INVOLVED IN INITIATION OF VESICULAR STOMATITIS-VIRUS RNA-TRANSCRIPTION INVITRO
UNIQUE RNA SPECIES INVOLVED IN INITIATION OF VESICULAR STOMATITIS-VIRUS RNA-TRANSCRIPTION INVITRO
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DOI：
10.1016/0092-8674(76)90003-9
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发表时间：
1976-01-01
期刊：
影响因子：
64.5
通讯作者：
BANERJEE, AK
中科院分区：
文献类型：
--
作者：
COLONNO, RJ;BANERJEE, AK
水泡性口炎病毒（VSV）的纯化病毒粒子能够在体外合成两种不同类型的病毒特异性RNA。第一种由几种病毒mRNA组成，这些mRNA先前显示含有封闭的5“末端序列GpppApApCpApGp和3”末端poly（A）。第二类RNA具有未封闭的5“末端，并且不包含足够长的聚（A）延伸以结合寡聚（dT）-纤维素柱。它在20%聚丙烯酰胺凝胶中迁移为单一均匀峰，估计链长为68个核苷酸。碱基分析表明，该小RNA分子由48%AMP、20%CMP、11%GMP和21%UMP组成。小RNA的5“末端序列是ppApCpGp，它似乎与VSV基因组RNA的3”末端序列互补（... PypGpU）。该小RNA分子可能代表起始的导入RNA片段，其在VSV mRNA形成期间通过可能的加工机制被去除。
Purified virions of vesicular stomatitis virus (VSV) are capable of synthesizing 2 distinct types of virus-specific RNA in vitro. The 1st consists of several viral mRNAs which were previously shown to contain the blocked 5'' terminal sequence GpppApApCpApGp and 3'' terminal poly(A). The 2nd type of RNA has an unblocked 5'' terminus and does not contain poly(A) stretches long enough to bind to oligo(dT)-cellulose columns. It migrates in 20% polyacrylamide gels as a single homogenous peak with an estimated chain length of 68 nucleotides. Base analysis demonstrated that this small RNA molecule is composed of 48% AMP, 20% CMP, 11% GMP and 21% UMP. The 5'' terminal sequence of the small RNA is ppApCpGp, which appears to be complementary to the 3'' terminal sequence of the VSV genome RNA (...PypGpU). This small RNA molecule probably represents the initiated lead-in RNA segment which is removed during formation of VSV mRNAs by a possible processing mechanism.