Balloon Catheter Injury to Rabbit Carotid Artery: I. Changes in Smooth Muscle Phenotype
Balloon Catheter Injury to Rabbit Carotid Artery: I. Changes in Smooth Muscle Phenotype
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DOI：
10.1161/01.atv.9.3.289
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发表时间：
1989-05
期刊：
影响因子：
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通讯作者：
J. A. Manderson;P. Mosse;Julie A. Safstrom;S. B. Young;G. Campbell
中科院分区：
文献类型：
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作者：
J. A. Manderson;P. Mosse;Julie A. Safstrom;S. B. Young;G. Campbell
应用体视学方法观察球囊导管损伤后血管内膜增厚形成过程中，血管内膜细胞超微结构的变化。在兔颈动脉的平滑肌细胞线区域(对Evans蓝染料可自由渗透，因此染成蓝色)和重新内皮化区(注入Evans蓝后仍为白色)，测量细胞质中肌纤维的体积密度。损伤后2周，白区和蓝区内膜细胞内肌膜的体积密度均显著低于对照组(67.9%±3.6%；均值±SE)，分别为38.8%±1.0%和35.9±3.3%。伤后6周，白区(55.1%±3.4%)和蓝区(53.5%±3.0%)的体密度均明显增加，18周时无明显变化。损伤后18周，蓝区管腔(衬里)平滑肌细胞内肌膜的体积密度明显低于对照组中膜细胞(26.7%±2.1%)，并持续到伤后18周。最初的球囊损伤对中膜的平滑肌细胞造成了相当大的损伤，其余的中层细胞在超微结构上的变化与肿瘤中的细胞相似。伤后2周，肌纤维的体积密度较低(44.9%±2.4%)，伤后6周恢复到与对照动脉无明显差异的水平。损伤动脉内、外中层肌纤维的体积密度估计值无差异。这些结果表明，球囊导管损伤后，中膜的平滑肌细胞和由此导致的内膜增厚在超微结构上发生了可逆的变化。
Stereology was used to Investigate the changes In ultrastructure of smooth muscle cells during the formation of an experimental Intimal thickening Induced by Injury with an Inflated balloon catheter. The volume density of myofllaments in the cell cytoplasm was measured In smooth muscle cell-lined areas (which are freely permeable to Evans blue dye and, hence, stain blue) and In re-endothellalized areas (which remain white after Injection of Evans blue) of the rabbit carotid artery. Two weeks after Injury, the volume densities of myofllaments In the Intimal smooth muscle cells In both white and blue areas were significantly less than that for control medial smooth muscle (67.9%±3.6%; mean±SE), being 38.8%±1.0%and 35.9±3.3%, respectively. By 6 weeks after injury, the volume density had Increased significantly In both white (55.1%±3.4%) and blue areas (53.5%±3.0%), and these values did not change significantly by 18 weeks. The volume density of myofllaments in the luminal (lining) smooth muscle cells In the blue areas was significantly less than that of control medial cells and remained low (26.7%±2.1%) up to 18 weeks after Injury. The Initial balloon-Induced Injury caused considerable damage to the smooth muscle cells In the media, and the remaining medial cells underwent similar changes In ultrastructure to the cells in the neointlma. At 2 weeks, the cells had a low volume density of myofllaments (44.9%±2.4%), which returned to a level not significantly different from the control artery by 6 weeks after Injury. There were no differences in the estimates of the volume density of myofllaments between the Inner and outer media of the Injurled arteries. These findings suggest that, after Injury produced by a balloon catheter, the smooth muscle cells In both the media and the resultant Intimal thickening undergo a reversible change In ultrastructure.