Extracellular generation of hydrogen peroxide is responsible for activation of EGF receptor by ultraviolet A radiation.
Extracellular generation of hydrogen peroxide is responsible for activation of EGF receptor by ultraviolet A radiation.
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细胞外产生的过氧化氢负责通过紫外线 A 辐射激活 EGF 受体。
DOI：
10.1016/j.freeradbiomed.2006.08.005
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发表时间：
2006
影响因子：
7.4
通讯作者：
L. Klotz
中科院分区：
文献类型：
--
作者：
Claudia von Montfort;Niklas S. Fernau;J. Beier;H. Sies;L. Klotz
受体酪氨酸激酶，如表皮生长因子受体(EGFR)，已被认为在紫外线A(UVA)辐射(320-400 nm)下被激活，并参与光癌的发生。单线态氧和过氧化氢被认为是UVA激活信号转导通路的介质。这表明，在没有细胞外光敏剂的情况下，暴露于UVA的细胞中不会激活EGFR。相反，UVA只有在允许细胞外光化学产生过氧化氢的条件下，才能激活HeLa细胞和人类角质形成细胞中的EGFR和相关的ErbB2受体酪氨酸激酶，例如当细胞在UVA暴露期间被细胞培养液覆盖时。UVA诱导的EGFR激活需要用钒酸盐预处理，这表明蛋白酪氨酸磷酸酶参与了该过程。与过氧化氢不同，光化学产生的单线态氧并不激活EGFR，而是通过其配体EGF削弱EGFR的激活。总之，细胞外产生的过氧化氢介导了UVA诱导的EGFR和ErbB2的激活，而细胞内暴露于UVA时产生的活性氧不足以激活受体。
Receptor tyrosine kinases such as the epidermal growth factor receptor (EGFR) have been proposed to be activated in cells exposed to ultraviolet A (UVA) radiation (320–400 nm) and to be involved in photocarcinogenesis. Singlet oxygen and hydrogen peroxide are being discussed as mediators of the activation of signal transduction pathways by UVA. It is demonstrated here that EGFR is not activated in cells exposed to UVA in the absence of extracellular photosensitizers. Rather, UVA was capable of activating the EGFR and the related ErbB2 receptor tyrosine kinase in HeLa cells and human keratinocytes only under conditions that allowed for the extracellular photochemical generation of H2O2, such as when cells were covered with cell culture medium during exposure to UVA. Pretreatment of cells with vanadate was required for UVA-induced EGFR activation, pointing to the involvement of protein tyrosine phosphatases. Unlike H2O2, photochemically generated singlet oxygen did not activate EGFR but instead impaired the activation of EGFR by its ligand, EGF. In summary, extracellularly generated H2O2mediates UVA-induced activation of the EGFR and of ErbB2, whereas intracellular generation of reactive oxygen species upon exposure of cells to UVA is not sufficient for activation of the receptor.
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影响因子：
11.2
作者：
El-Abaseri, TB;Fuhrman, J;Hansen, LA
通讯作者：
Hansen, LA
通过紫外线 A 照射诱导 EGFR 依赖性和 EGFR 非依赖性信号通路。
影响因子：
3.1
作者：
Zhang,Y;Dong,Z;Bode,AM;Ma,WY;Chen,N;Dong,Z
通讯作者：
Dong,Z
DOI：
10.1073/pnas.0501154102
发表时间：
2005-04-05
影响因子：
11.1
作者：
DeYulia, GJ;Cárcamo, JM;Golde, DW
通讯作者：
Golde, DW
影响因子：
4.7
作者：
El-Abaseri, TB;Putta, S;Hansen, LA
通讯作者：
Hansen, LA
大麻素抑制大鼠肝上皮细胞系中间隙连接细胞间通讯并激活 ERK。
影响因子：
6.4
作者：
Upham,BradL;Rummel,AlisaM;Carbone,JosephM;Trosko,JamesE;Ouyang,Yanli;Crawford,RobertB;Kaminski,NorbertE
通讯作者：
Kaminski,NorbertE