Culture of oocytes and risk of imprinting defects
Culture of oocytes and risk of imprinting defects
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DOI：
10.1093/humupd/dms042
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发表时间：
2013-01-01
影响因子：
13.3
通讯作者：
Smitz, Johan
中科院分区：
文献类型：
--
作者：
Anckaert, Ellen;De Rycke, Martine;Smitz, Johan
卵泡培养和卵母细胞体外成熟（IVM）是新兴的辅助生殖技术，在生育临床中具有潜在的重要应用前景。人们担心这些技术可能会在表观遗传水平上产生干扰，特别是对基因组印记产生干扰。及时获得正确的卵母细胞印迹模式和受精后基因组印迹的维持都是胚胎正常发育所必需的。在Pubmed中进行了系统的文献检索，保留了所有报道卵泡培养、IVM或卵巢组织培养对基因组印迹影响的文献。小鼠卵巢组织培养研究，小鼠体外卵泡培养研究和单一牛IVM研究通常显示卵母细胞中正确的印迹DNA甲基化建立。在小鼠卵泡培养中，处理和次优培养条件的影响表明，在卵母细胞中建立印迹是一个稳健的过程。这与在小鼠中报道的植入前胚胎培养诱导的表观遗传缺陷形成对比。对于人类IVM，由于设计良好的研究目前尚不可用，因此无法得出关于印迹建立的明确结论。动物模型提供了培养卵母细胞中印迹建立的可靠数据，但进一步的研究应评估卵母细胞培养对印迹维持的影响。应在设计良好的人体研究中评估优化的IVM程序。最后，应在IVM后妊娠所生的儿童中进行表观遗传分析，以得出关于人类IVM表观遗传安全性的明确结论。
Follicle culture and oocyte in vitro maturation (IVM) are emerging assisted reproductive technologies with potentially important future applications in the fertility clinic. There is concern that these technologies might interfere at the epigenetic level and, in particular, with genomic imprinting. The timely acquisition of correct imprinting patterns in oocytes and the maintenance of genomic imprinting after fertilization are both required for normal embryonic development.A systematic literature search in Pubmed was performed and all publications reporting on the effects of follicle culture, IVM or ovarian tissue culture on genomic imprinting were retained.Mouse ovarian tissue culture studies, mouse in vitro follicle culture studies and a single bovine IVM study generally showed correct imprinted DNA methylation establishment in oocytes. Influences of treatment and suboptimal culture conditions in mouse follicle culture indicate that imprinting establishment in oocytes is a robust process. This is in contrast with preimplantation embryo culture-induced epigenetic defects reported in mice. For human IVM, no definitive conclusion on imprinting establishment can be drawn as well-designed studies are currently not available.Animal models provide reassuring data on imprinting establishment in cultured oocytes, but further studies should assess the effect of oocyte culture on imprinting maintenance. Optimized IVM procedures should be assessed in well-designed human studies. Finally, epigenetic analysis should be performed in children born from pregnancies after IVM to draw definitive conclusions on the epigenetic safety of human IVM.