2 YEAST GENES WITH SIMILARITY TO TCP-1 ARE REQUIRED FOR MICROTUBULE AND ACTIN FUNCTION IN-VIVO
2 YEAST GENES WITH SIMILARITY TO TCP-1 ARE REQUIRED FOR MICROTUBULE AND ACTIN FUNCTION IN-VIVO
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DOI：
10.1073/pnas.91.19.9111
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发表时间：
1994-09-13
影响因子：
11.1
通讯作者：
HUFFAKER, TC
中科院分区：
文献类型：
--
作者：
CHEN, XY;SULLIVAN, DS;HUFFAKER, TC
我们已经分离出两个基因的酵母酿酒酵母，BIN 2和BIN 3，导致异常染色体分离在体内的冷敏感突变。BIN 2和BIN 3编码彼此相似且与TCP-1相似的必需蛋白质。TCP-1和TCP-1样蛋白是真核细胞质伴侣蛋白的组分，其促进微管蛋白和肌动蛋白在体外的折叠。BIN 2和BIN 3的突变导致体内微管和肌动蛋白组装的缺陷，并赋予对微管不稳定药物苯菌灵的超敏感性。TCP-1家族的第四个成员TCP 1、BIN 2、BIN 3或ANC 2在酵母中的过表达并不与其他基因的突变互补，这表明这些蛋白质具有不同的功能。然而，所有的双突变体组合都是无效的;这种合成致死性表明蛋白质在一个共同的过程中起作用。这些结果表明，Bin 2 p和Bin 3 p是酵母细胞质伴侣蛋白复合物的组分，该复合物是体内微管和肌动蛋白组装所需的。
We have isolated cold-sensitive mutations in two genes of the yeast Saccharomyces cerevisiae, BIN2 and BIN3, that cause aberrant chromosome segregation in vivo. BIN2 and BIN3 encode essential proteins that are similar to each other and to TCP-1. TCP-1 and TCP-1-like proteins are components of the eukaryotic cytoplasmic chaperonin that facilitates folding of tubulins and actin in vitro. Mutations in BIN2 and BIN3 cause defects in microtubule and actin assembly in vivo and confer supersensitivity to the microtubule-destabilizing drug benomyl. Overexpression of TCP1, BIN2, BIN3, or ANC2, a fourth member of the TCP-1 family in yeast, does not complement mutations in the other genes, indicating that the proteins have distinct functions. However, all double-mutant combinations are inviable; this synthetic lethality suggests that the proteins act in a common process. These results indicate that Bin2p and Bin3p are components of a yeast cytoplasmic chaperonin complex that is required for assembly of microtubules and actin in vivo.