A simple route to functionalize polyacrylamide hydrogels for the independent tuning of mechanotransduction cues
A simple route to functionalize polyacrylamide hydrogels for the independent tuning of mechanotransduction cues
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DOI：
10.1039/c2lc41168g
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发表时间：
2013-01-01
期刊：
影响因子：
6.1
通讯作者：
Gabriele, Sylvain
中科院分区：
文献类型：
--
作者：
Grevesse, Thomas;Versaevel, Marie;Gabriele, Sylvain
已知局部细胞微环境中的物理化学和生化因素通过特定的信号通路影响细胞行为的多个方面。这些机械转导信号可以相互耦合来调节细胞命运，但不同环境下的机械转导是否有共同的机制尚不清楚。毫无疑问，一项挑战将涉及进一步确定这种合作机制的特征，以及明确界定每个机械和生化参数的个别作用。为了以独立的方式控制这些机械转导线索，我们开发了一种简单有效的策略来固定聚丙烯酰胺水凝胶上任何所需性质的蛋白质，并独立控制细胞微环境的各种参数，如基质刚度，细胞结合配体密度和受限粘附性。这种新颖的平台通过单细胞实验得到验证，为研究机械转导过程中复杂的相互作用开辟了广阔的途径。
Physico-chemical and biochemical factors in the local cellular microenvironment are known to impact on multiple aspects of cell behaviour through specific signal pathways. These mechanotransduction cues can couple each other to regulate cell fate, and it remains unclear whether mechanotransduction in different contexts shares common mechanisms. Undoubtedly, a challenge will involve the further characterization of such cooperative mechanisms, as well as clearly defining the individual role of each mechanical and biochemical parameter. To control these mechanotransduction cues in an independent manner, we developed a simple and efficient strategy to immobilize any desired nature of proteins on polyacrylamide hydrogels and independently control various parameters of the cell microenvironment, such as matrix stiffness, cell-binding ligand density and confined adhesiveness. This novel platform is validated by conducting single-cell experiments and opens a broad avenue for studying complex interplays involved in mechanotransduction with a facile and versatile approach.