Kinetic capillary electrophoresis (KCE): A conceptual platform for kinetic homogeneous affinity methods
Kinetic capillary electrophoresis (KCE): A conceptual platform for kinetic homogeneous affinity methods
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DOI：
10.1021/ja056232l
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发表时间：
2005-12-07
影响因子：
15
通讯作者：
Krylov, SN
中科院分区：
文献类型：
--
作者：
Petrov, A;Okhonin, V;Krylov, SN
我们提出动态毛细管电泳（KCE）作为一个概念平台，为发展的动力学均相亲和方法。KCE被定义为电泳过程中相互作用的物质的CE分离。根据如何安排交互，可以设计不同的KCE方法。所有的KCE方法都用相同的数学来描述：相同的偏微分方程组，只是初始条件和边界条件不同。每一个定性唯一的初始条件和边界条件集合定义了唯一的KCE方法。在这里，我们(i)提出了KCE的理论基础，（ii）定义了四种新的KCE方法，（iii）提出了一个多方法的KCE工具箱作为一个集成的动力学技术。使用KCE工具箱，我们首次能够观察到同一蛋白质-配体对内的高亲和力（特异性）和低亲和力（非特异性）相互作用。KCE的概念允许创建强大的动力学均相亲和方法的扩展工具集，这将在生物分子相互作用的研究中找到它们的应用，定量分析，从复杂混合物中选择亲和探针和候选药物。
We propose kinetic capillary electrophoresis (KCE) as a conceptual platform for the development of kinetic homogeneous affinity methods. KCE is defined as the CE separation of species that interact during electrophoresis. Depending on how the interaction is arranged, different KCE methods can be designed. All KCE methods are described by the same mathematics: the same system of partial differential equations with only initial and boundary conditions being different. Every qualitatively unique set of initial and boundary conditions defines a unique KCE method. Here, we (i) present the theoretical bases of KCE, (ii) define four new KCE methods, and (iii) propose a multimethod KCE toolbox as an integrated kinetic technique. Using the KCE toolbox, we were able to, for the first time, observe high-affinity (specific) and low-affinity (nonspecific) interactions within the same protein-ligand pair. The concept of KCE allows for the creation of an expanding toolset of powerful kinetic homogeneous affinity methods, which will find their applications in studies of biomolecular interactions, quantitative analyses, and selecting affinity probes and drug candidates from complex mixtures.