Anti-inflammatory effect of bee venom on antigen-induced arthritis in rabbits: Influence of endogenous glucocorticoids
Anti-inflammatory effect of bee venom on antigen-induced arthritis in rabbits: Influence of endogenous glucocorticoids
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DOI：
10.1016/j.jep.2010.04.015
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发表时间：
2010-07-06
影响因子：
5.4
通讯作者：
Verissimo de Mello, Suzana Beatriz
中科院分区：
文献类型：
--
作者：
Freire Saad Rached, Izabella Cordeiro;Castro, Fabio Morato;Verissimo de Mello, Suzana Beatriz
研究目的：本研究探讨了内源性糖皮质激素（GC）在蜂毒（BV）抗兔抗原诱导性关节炎（AIA）中的作用。白细胞总数和分类计数。检测关节液中PGE（2）水平和滑膜细胞浸润情况。在用BV加甲吡酮（GC合成抑制剂）或RU-38 486（类固醇拮抗剂）治疗的家兔中评估GC对BV作用的贡献。BV治疗（1.5 μ g/kg/天）降低白细胞计数和PGE 2水平（分别为18571 +/- 1909个细胞/mm（3）和0.49 +/- 0.05 ng/mL）以及与对照组相比的细胞浸润（40968 +/-5248个细胞/mm（3）和2.92 +/-0.68 ng/mL，p < 0.05）。此外，甲吡酮BV治疗完全逆转了AIA的抑制，而RU-38 486是无效的。结论：我们的数据表明，蜂毒治疗防止兔抗原诱导的关节炎的发展，通过GC的作用。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Aim of the study: This study assessed the involvement of endogenous glucocorticoids (GCs) in the anti-arthritic properties of bee venom (BV) on antigen-induced arthritis (AIA) in rabbits.Materials and methods: BV (1.5-6 mu g/kg/day) was injected for 7 days before AIA induction, whereas the control group received sterile saline. The total and differential leukocyte count. PGE(2) levels in synovial fluid and synovial membrane cell infiltrate were evaluated. The contribution of GCs to BV action was assessed in rabbits treated with BV plus metyrapone, an inhibitor of GC synthesis, or RU-38 486, a steroid antagonist.Results: Treatment with BV (1.5 mu g/kg/day) reduced the leukocyte count and PGE2 level (18571 +/- 1909 cells/mm(3) and 0.49 +/- 0.05 ng/mL, respectively) as well as the cellular infiltrate compared with the control group (40968 +/- 5248 cells/mm(3) and 2.92 +/- 0.68 ng/mL, p < 0.05). The addition of metyrapone to BV treatment completely reversed the inhibition of AIA, whereas RU-38 486 was ineffective.Conclusion: Our data show that bee venom treatment prevents the development of antigen-induced arthritis in rabbits through the action of GCs. (C) 2010 Elsevier Ireland Ltd. All rights reserved.