Genomic changes associated with antigenic variation of visna virus durig persistent infection.
Genomic changes associated with antigenic variation of visna virus durig persistent infection.
复制标题
持续感染期间与维斯纳病毒抗原变异相关的基因组变化。
DOI：
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发表时间：
1980
影响因子：
11.1
通讯作者：
W. Haseltine
中科院分区：
文献类型：
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作者：
J. Clements;F. Pedersen;O. Narayan;W. Haseltine
绵羊持续感染维斯纳病毒时，病毒抗原发生变化。通过使用基因组RNA和分析大RNase T1抗性寡核苷酸来比较visna病毒的抗原变体。从持续感染的绵羊中分离的突变体与亲本病毒相比，其寡核苷酸模式有少量变化。为了确定核苷酸结构的变化是否聚集在基因组的一个区域中，我们确定了亲本和突变体RNA的寡核苷酸沿着基因组相对于3'聚腺苷酸化末端的顺序。亲本菌株和用于作图的抗原突变体之间的所有差异（除了一个差异）位于距3'末端的2个内切酶内。核苷酸序列分析表明，在亲本和突变体RNA中不同的寡核苷酸中的几个可以通过单碱基变化来解释。
Visna virus undergoes antigenic change during persistent infection of sheep. Antigenic variants of visna virus were compared by using the genomic RNA and analyzing the large RNase T1-resistant oligonucleotides. Mutants isolated from a persistently infected sheep contained a small number of changes in their oligonucleotide patterns when compared with parental virus. To determine whether the changes in the nucleotide structure were clustered in one region of the genome, we determined the order of the oligonucleotides of the parental and mutant RNAs along the genome with respect to the 3' polyadenylylated end. All but one difference between the parental strain and the antigenic mutant used for mapping were located within 2 kilobases from the 3' terminus. Nucleotide sequence analyses showed that several of the oligonucleotides that differed in the parental and mutant RNAs could be accounted for by single base changes.