CYTO-TOXICITY OF DENTAL COMPOSITES AND OTHER MATERIALS IN A NEW INVITRO DEVICE
CYTO-TOXICITY OF DENTAL COMPOSITES AND OTHER MATERIALS IN A NEW INVITRO DEVICE
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DOI：
10.1111/j.1600-0714.1988.tb01304.x
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发表时间：
1988-09-01
影响因子：
3.3
通讯作者：
PASHLEY, DH
中科院分区：
文献类型：
--
作者：
HANKS, CT;CRAIG, RG;PASHLEY, DH
多年来，已经开发了几种体外方法来改善牙科修复材料筛选试验与使用试验之间的相关性。在使用试验中，牙髓对恢复性材料的反应通常没有细胞在体外筛选试验或植入试验中（实验材料与重要细胞之间有直接接触）的反应严重。在本研究测试的“体外牙髓室”装置中，牙本质圆盘散布在复合树脂（以及其他牙科材料）和培养基之间，作为Balb/c 3T3测试细胞系统的营养来源。牙本质限制了物质进入“牙髓腔”的扩散到临床相关的水平。光固化复合树脂（complete和P30），当放置在0.5 mm而不是1.5 mm厚的牙本质培养皿上时，仅在聚合后的前24小时内引起蛋白质合成的抑制。与阴性对照相比，化学固化的复合材料（SILAR和P10）在任何聚合后时间，在0.5 mm或1.5 mm的牙本质圆盘上都没有明显的蛋白质合成抑制作用。
Several in vitro methods have been developed over the years to improve correlation between screening tests and usage tests of dental restorative materials. Pulpal responses to restorative materials in usage tests are usually less severe than are responses of cells either in vitro screening tests or in implantation tests where there is direct contact between experimental materials and vital cells. In the "in vitro pulp chamber" device tested in the present study, dentin disks were interspersed between composite resins (as well as other dental materials) and the medium which served as the nutrient source for the Balb/c 3T3 test cell system. The dentin restricted the diffusion of materials into the "pulp chamber" to clinically relevant levels. The light-cured composite resins (Fulfil and P30), caused depression of protein synthesis only during the first 24 h postpolymerization, when placed on 0.5 mm but not 1.5 mm thick dentin dishes. The chemically-set composites (SILAR and P10) caused no significant inhibition of protein synthesis (as compared to negative controls) at any postpolymerization time on either 0.5 mm or 1.5 mm dentin disks.