The role of activation-induced cytidine deaminase (AID/AICDA) in the progression of follicular lymphoma
The role of activation-induced cytidine deaminase (AID/AICDA) in the progression of follicular lymphoma
复制标题
激活诱导胞苷脱氨酶（AID/AICDA）在滤泡性淋巴瘤进展中的作用
DOI：
10.1111/j.1349-7006.2011.02186.x
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发表时间：
2012
期刊：
影响因子：
5.7
通讯作者：
et al
中科院分区：
文献类型：
--
作者：
Shikata H;Yasukawa M;et al
激活诱导的胞苷脱氨酶 (AID/AICDA) 是免疫球蛋白基因的体细胞超突变和类别转换重组以及生发中心来源的 B 细胞淋巴瘤的 forc-myc 易位所必需的。在本研究中，我们试图利用 RT-PCR 和定量实时 PCR 阐明 AID 与 c-mycin 相关的滤泡性淋巴瘤 (FL) 进展的重要性。 3 级 FL 患者的组织表达相对较高水平的 c-mycandAID。从开始治疗后 2 年内死亡的 FL 患者采集的样本显示，尽管 c-myc 表达水平很高，但 AID 没有表达或表达较低。为了检查 AID 表达在快速进展性 FL 中的作用，将全长 AID 转录物转染到由不同快速进展性 FL 患者建立的 AID 阴性细胞系中。这导致表达 AID 的转染子的建立，与对照相比，其增殖率低且 G0/G1 停滞发生率显着增加。我们的结果表明，当 c-mycis 表达充足时，AID 可能充当 FL 细胞存活的负调节因子。 mycamplification 后 AID 的关闭或低表达可能与 FL 的临床结果相关。 （癌症科学2012；103：415–421）
Activation‐induced cytidine deaminase (AID/AICDA) is required for somatic hypermutation and class‐switch recombination of the immunoglobulin gene, and forc‐myctranslocation of germinal center‐derived B‐cell lymphoma. In the present study, we attempted to clarify the significance ofAIDassociated withc‐mycin the progression of follicular lymphoma (FL) using RT‐PCR and quantitative real‐time PCR. Tissues from the patients with grade 3 FL expressed relatively higher levels ofc‐mycandAID. The samples taken from a patient with FL who died within 2 years after the start of treatment showed either no or low expression ofAID, despite expressing high levels ofc‐myc. In order to examine the role of AID expression in rapidly progressive FL, the full‐lengthAIDtranscript was transfected intoAID‐negative cell lines established from different patients with rapidly progressive FL. This led to the establishment ofAID‐expressing transfectants with a low proliferation rate and a significantly increased incidence of G0/G1arrest compared with controls. Our results indicate that AID may act as a negative regulator of cell survival in FL when sufficientc‐mycis expressed. Switch‐off or low expression ofAIDafterc‐mycamplification may correlate with the clinical outcomes of FL. (Cancer Sci2012; 103: 415–421)