INTRACELLULAR MOVEMENTS OF FLUORESCENTLY LABELED SYNAPTIC VESICLES IN FROG MOTOR-NERVE TERMINALS DURING NERVE-STIMULATION
INTRACELLULAR MOVEMENTS OF FLUORESCENTLY LABELED SYNAPTIC VESICLES IN FROG MOTOR-NERVE TERMINALS DURING NERVE-STIMULATION
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DOI：
10.1016/0896-6273(92)90235-6
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发表时间：
1992-11-01
期刊：
影响因子：
16.2
通讯作者：
RIDGE, RMAP
中科院分区：
文献类型：
--
作者：
BETZ, WJ;BEWICK, GS;RIDGE, RMAP
我们用FM1-43对青蛙运动神经末梢的突触小泡进行了染色，并研究了神经刺激过程中小泡团的形状和位置的变化。每个染色的囊泡团均显示为一个荧光点。在重复的神经刺激过程中，这些斑点逐渐变暗，大多数不改变形状或位置。然而，偶尔会有一个斑点移动，在某些情况下似乎向邻近的斑点流动并与其合并。这表明存在能够在囊泡团之间以协调的方式主动移动小泡的易位机制。在单簇中，我们没有看到这种定向的囊泡运动的迹象。在共聚焦显微镜下观察，在刺激神经时，终末的荧光点并没有向突触前膜缩小，而是均匀地变暗。这表明，在脱色过程中，小泡在簇内持续混合，没有证据表明单个小泡簇内有活跃的小泡转运体将小泡移动到突触前膜。
We stained synaptic vesicles in frog motor nerve terminals with FM1-43 and studied changes in the shape and position of vesicle clusters during nerve stimulation. Each stained vesicle cluster appeared as a fluorescent spot. During repetitive nerve stimulation the spots gradually dimmed, most without changing shape or position. Occasionally, however, a spot moved, appearing in some cases to stream toward and coalesce with a neighboring spot. This suggests the existence of translocation mechanisms that can actively move vesicles in a coordinated fashion between vesicle clusters. Within single clusters, we saw no signs of such directed vesicle movements. Flourescent spots in terminals viewed from the side with a confocal microscope did not shrink toward the presynaptic membrane during nerve stimulation, but dimmed uniformly. This suggests that vesicles continuously mix within a cluster during destaining and provides no evidence of active vesicle translocators within single vesicle clusters for moving vesicles to the presynaptic membrane.