Effect of channel geometry on cell adhesion in microfluidic devices
Effect of channel geometry on cell adhesion in microfluidic devices
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DOI：
10.1039/b813516a
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Murthy, Shashi K.
中科院分区：
文献类型：
--
作者：
Green, James V.;Kniazeva, Tatiana;Murthy, Shashi K.
用配体包被的微流体通道是用于从小样品体积中分离或富集细胞的通用平台。这种基于粘附的分离模式由细胞和通道表面之间的配体受体键以及流体剪切应力介导。本文演示了如何方面的微通道几何形状可以发挥额外的作用，在控制细胞粘附。结合计算流体动力学建模和细胞粘附实验，具有急转弯的通道被示出在转弯区域具有接近零速度的区域，在转弯区域大量细胞粘附或被收集。与这些通道的其他区域相比，在转弯区域中缺乏均匀的粘附，以及观察到的细胞粘附的大的可变性表明，具有急转弯的通道对于期望可预测的细胞粘附的细胞捕获应用不是最佳的。另一方面，具有弯曲转弯的通道显示出提供更均匀和可预测的细胞粘附，只要通道的平行臂之间的差距足够宽。这些弯曲通道中的细胞粘附的幅度与没有转弯的直通道中的细胞粘附的幅度相当。
Microfluidic channels coated with ligands are a versatile platform for the separation or enrichment of cells from small sample volumes. This adhesion-based mode of separation is mediated by ligand receptor bonds between the cells and channel surface and also by fluid shear stress. This paper demonstrates how aspects of microchannel geometry can play an additional role in controlling cell adhesion. With a combination of computational fluid dynamics modeling and cell adhesion experiments, channels with sharp turns are shown to have regions with near-zero velocity at the turn regions where large numbers of cells adhere or become collected. The lack of uniform adhesion in the turn regions compared to other regions of these channels, together with the large variability in observed cell adhesion indicates that channels with sharp turns are not optimal for cell-capture applications where predictable cell adhesion is desired. Channels with curved turns, on the other hand are shown to provide more uniform and predictable cell adhesion provided the gap between parallel arms of the channels is sufficiently wide. The magnitude of cell adhesion in these curved channels is comparable to that in straight channels with no turns.