Compensatory base changes illuminate morphologically difficult taxonomy.
Compensatory base changes illuminate morphologically difficult taxonomy.
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DOI：
10.1016/j.ympev.2009.07.036
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发表时间：
2010-02
影响因子：
4.1
通讯作者：
Michael W. Ruhl;M. Wolf;T. Jenkins
中科院分区：
文献类型：
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作者：
Michael W. Ruhl;M. Wolf;T. Jenkins
核糖体RNA （rRNA）内部转录间隔2 （ITS2）二级结构的代偿性碱基变化（CBCs）已被成功用于验证近缘物种的分类。CBCs从未用于区分形态模糊的物种。基于CBC可以区分高等真核生物物种的假设，将CBC分析软件应用于Altica属中形态上难以区分的昆虫物种。该分析是对在四个生态区收集的同域Altica甲虫的物种特异性分析，并与扫描电子显微镜数据一致。本研究表明，在ITS2 rRNA二级结构中挖掘CBCs是真核生物分类群分析的有效方法。
Compensatory base changes (CBCs) in the ribosomal RNA (rRNA) internal transcribed spacer 2 (ITS2) secondary structures have been used to successfully verify the taxonomy of closely related species. CBCs have never been used to distinguish morphologically indistinct species. Under the hypothesis that CBCs will differentiate species in higher eukaryotes, novel software for CBC analysis was applied to morphologically indistinguishable insect species in the genus Altica. The analysis was species-specific for sympatric Altica beetles collected across four ecoregions and concordant with scanning electron microscopy data. This research shows that mining for CBCs in ITS2 rRNA secondary structures is an effective method for eukaryotic taxon analysis.