Tissue In Situ Hybridization: Methods in Animal Development
Tissue In Situ Hybridization: Methods in Animal Development
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组织原位杂交：动物发育方法
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
T. Jowett
中科院分区：
文献类型：
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作者：
T. Jowett
技术的原则。探针合成。固定。杂交到整体-安装胚胎。探针的免疫定位。替代酶底物。双色原位杂交。荧光染料双色原位杂交。包埋和切片整个-安装胚胎。石蜡包埋组织切片上的原位杂交。鸡胚冷冻切片的原位杂交。厚的漂浮组织切片的整体原位杂交。转录和翻译基因产物的同时定位。特殊注意事项、修改和故障排除。显微摄影。溶液、试剂、设备和供应商。参考资料。指数.
Principle of the Technique. Probe Synthesis. Fixation. Hybridization to Whole--Mount Embryos. Immunolocalization of Probes. Alternative Enzyme Substrates. Two--Color In Situ Hybridizations. Two--Color In Situ Hybridization with Fluorochromes. Embedding and Sectioning Whole--Mount Embryos. In Situ Hybridization on Paraffin Wax Embedded Tissue Sections. In Situ Hybridization on Cryostat Sections of Chick Embryos. Whole--Mount In Situ Hybridization of Thick, Floating Tissue Sections. Simultaneous Localization of Transcription and Translation Gene Products. Special Considerations, Modifications, and Problem Troubleshooting. Photomicrography. Solutions, Reagents, Equipment, and Suppliers. References. Index.