Components and Properties of the G3 Ganglion Cell Circuit in the Rabbit Retina
Components and Properties of the G3 Ganglion Cell Circuit in the Rabbit Retina
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DOI：
10.1002/cne.21941
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发表时间：
2009-03-01
影响因子：
2.5
通讯作者：
Mills, Stephen L.
中科院分区：
文献类型：
--
作者：
Hoshi, Hideo;Mills, Stephen L.
视网膜上的每一点都由大约15种类型的神经节细胞采样，每一种神经节细胞都是回路中的一个元件，还包含特定类型的双极细胞和无长突细胞。这些电路中只有一小部分得到了很好的表征。我们发现，细胞内注射神经生物素到一个特定的神经节细胞类型的荧光标记物的目标也染色不对称分支神经节细胞。它也被示踪剂偶联到一种不寻常类型的无长突细胞，其树突强烈不对称，仅在背侧方向从索马以窄束行进。神经节细胞的树突场最初分层在sublamina B（ON层），但很少有专门化和分支，然后更广泛地在sublamina a（OFF层）在耦合的无长突细胞的过程的水平。神经节和无长突细胞过程的交叉点含有Cx 36免疫阳性的斑点。此外，我们发现多巴胺能无长突细胞与神经节细胞和无长突细胞都有接触，并且钙结合蛋白突触的双极细胞免疫阳性到神经节细胞的sublamina B过程上。多巴胺D-1受体激活减少示踪剂流到无长突细胞。因此，我们靶向并表征了两种知之甚少的视网膜细胞类型，并将它们与其他两种细胞类型放在一起。新视网膜回路的重要部分。这种独特的电路包括在神经节细胞和无长突细胞树突领域的明显的不对称性可能会导致大量的取向偏差。神经学比较杂志513：69-82，2009. (C)2008 Wiley-Liss，Inc.
Each point on the retina is sampled by about 15 types of ganglion cell, each of which is an element in a circuit also containing specific types of bipolar cell and amacrine cell. Only a few of these circuits are well characterized. We found that intracellular injection of Neurobiotin into a specific ganglion cell type targeted by fluorescent markers also stained an asymmetrically branching ganglion cell. It was also tracer-coupled to an unusual type of amacrine cell whose dendrites were strongly asymmetric, coursing in a narrow bundle from the soma in the dorsal direction only. The dendritic field of the ganglion cell stratifies initially in sublamina b (the ON layers), but with few specializations and branches, and then more extensively in sublamina a (the OFF layers) at the level of the processes of the coupled amacrine cell. Intersections of the ganglion and amacrine cell processes contain puncta immunopositive for Cx36. Additionally, we found that the dopaminergic amacrine cell makes contact with both the ganglion cell and the amacrine cell, and that a bipolar cell immunopositive for calbindin synapses onto the sublamina b processes of the ganglion cell. Dopamine D-1 receptor activation reduced tracer flow to the amacrine cells. We have thus targeted and characterized two poorly understood retinal cell types and placed them with two other cell types. in a substantial portion of a new retinal circuit. This unique circuit comprised of pronounced asymmetries in the ganglion cell and amacrine cell dendritic fields may result in a substantial orientation bias. J. Comp. Neurol. 513:69-82, 2009. (C) 2008 Wiley-Liss, Inc.