THE 2 PATTERNS OF REACTIVE ASTROCYTOSIS IN POSTISCHEMIC RAT-BRAIN
THE 2 PATTERNS OF REACTIVE ASTROCYTOSIS IN POSTISCHEMIC RAT-BRAIN
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DOI：
10.1038/jcbfm.1990.141
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发表时间：
1990-11-01
影响因子：
6.3
通讯作者：
LESSER, ML
中科院分区：
文献类型：
--
作者：
PETITO, CK;MORGELLO, S;LESSER, ML
在大鼠中研究了缺血后星形胶质细胞肥大和增生的分布和时间过程以及与神经元存活或坏死的关系，这些大鼠在颈动脉和椎动脉闭塞30分钟后再灌注3小时至5周。通过电子显微镜评价中间丝（IF）。免疫组化法测定IF蛋白，[3H]胸苷摄取法测定星形胶质细胞分裂。胶质细胞酸性蛋白（GFAP）在受损和未受损的大脑区域增加2天，并与细胞扩大增加IF，和转化GFAP阴性胶质细胞成GFAP阳性。细胞肥大和增加GFAP持续只有在区域的神经元坏死，而GFAP阳性星形胶质细胞的数量和大小恢复到控制水平，在未受损的地区2周。星形胶质细胞增生直到第3天才被观察到，并且仅限于受损的脑区域。波形蛋白阳性星形胶质细胞在2天内在受损的大脑中大量存在，并且仅在5周时保留在这些区域中。这些数据表明，反应性星形细胞增多症在未受损的大脑中发展，但随着生存期的延长是可逆的，而伴随结构性脑损伤的反应性星形细胞增多症持续时间延长，并与增生以及肥大相关。此外，研究结果表明，星形胶质细胞表达的波形蛋白是更具体的比GFAP在识别区域的永久性缺血损伤在早期postischemic期间。
The distribution and time course of postischemic astrocyte hypertrophy and hyperplasia and the relationship to neuronal viability or necrosis was studied in rats subjected to 30 min of carotid and vertebral artery occlusion followed by reperfusion from 3 h to 5 weeks. Intermediate filaments (IFs) were evaluated by electron microscopy. IF proteins by immunohistochemistry, and astrocyte division by [3H]thymidine uptake. Glial fibrillary acidic protein (GFAP) increased in damaged and nondamaged brain regions by 2 days and was associated with cell enlargement increases in IF, and transformation of GFAP-negative into GFAP-positive glia. Cell hypertrophy and increased GFAP persisted only in regions of neuronal necrosis whereas the number and size of GFAP-positive astrocytes returned to control levels in nondamaged regions by 2 weeks. Astrocyte hyperplasia was not seen until 3 days and was confined to damaged brain regions. Vimentin-positive astrocytes were numerous by 2 days in damaged brain and remained only in those regions at 5 weeks. The data demonstrate that reactive astrocytosis develops in undamaged brain, but is reversible with prolonged survival, whereas reactive astrocytosis that accompanies structural brain damage persists for prolonged periods and is associated with hyperplasia, as well as hypertrophy. In addition, the results show that astrocyte expression of vimentin is more specific than GFAP in identifying regions of permanent ischemic injury during the early postischemic period.