Role of lipid peroxidation in the pathogenesis of myopic and senile cataract
Role of lipid peroxidation in the pathogenesis of myopic and senile cataract
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DOI：
10.1136/bjo.80.9.840
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发表时间：
1996-09-01
影响因子：
4.1
通讯作者：
Cardia, L
中科院分区：
文献类型：
--
作者：
MicelliFerrari, T;Vendemiale, G;Cardia, L
目的/背景 - 最近在白内障晶状体中观察到了自由基的产生，抗氧化剂的消耗以及不饱和脂质的氧化，并且已经提出了视网膜在人白内生生成中的积极参与。为了验证这一假设，在玻璃体中和患有衰老或近视性cataract.methods的玻璃体和患者中测定了晶状体和丙二醛（MDA）水平的总（GSH）和氧化（GSSG）谷胱甘肽浓度（MDA）水平（MDA）水平。该研究是对34个镜头（核和epinucleus）（9个透明镜片，，， 14个带有特发性老年性白内障的镜片，有11个受严重近视性白内障影响的镜片），玻璃体为19个（7个非侧向，七个近视和五个对照）。按照芦苇的方法进行了谷胱甘肽的测定，而使用Dahle方法的修饰进行了丙二醛。反向透镜与透明镜头相比，白内障透镜显示GSH含量降低，GSSG的浓度升高。与老年性白内障相比，在近视白内障中发现了GSH的氧化消耗更高。同样，与对照和老年患者相比，在白内障镜头和近视患者的玻璃体中都观察到MDA水平的升高。结论 - 观察到的改变强烈表明视网膜脂质过氧化可能在人白内术中起关键作用，尤其是在近视类型。
Aims/background - Increased production of free radicals, consumption of antioxidant, and oxidation of unsaturated lipids have been observed recently in cataractous lenses and active participation of the retina in human cataractogenesis has been proposed. To verify this hypothesis, the total (GSH) and oxidised (GSSG) glutathione concentrations were assayed in the lens and the malondialdehyde (MDA) levels assayed in the vitreous and in the lens of normal controls and patients with senile or myopic cataract.Methods - The study was conducted on 34 lenses (nucleus and epinucleus) (nine clear lenses, 14 lenses with idiopathic senile cataract, and 11 lenses affected by severe myopic cataract) and vitreous of 19 (seven non-myopic, seven myopic, and five control) subjects. Glutathione determination was performed following the method of Reed, while malondialdehyde was assayed using a modification of the method of Dahle.Results - Cataractous lenses showed a decreased content of GSH and increased concentration of GSSG compared with clear lenses. A higher oxidative consumption of GSH was found in myopic cataracts compared with senile ones. Also, increased levels of MDA were observed both in cataractous lenses and in the vitreous of myopic patients compared with the control and the senile ones.Conclusion - The observed alterations strongly suggest that retinal lipid peroxidation might play a key role in human cataractogenesis, especially in the myopic type.