Two novel cationic antifungal peptides isolated from Bacillus pumilus HN-10 and their inhibitory activity against Trichothecium roseum
Two novel cationic antifungal peptides isolated from Bacillus pumilus HN-10 and their inhibitory activity against Trichothecium roseum
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DOI：
10.1007/s11274-017-2392-5
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发表时间：
2018-01
影响因子：
4.1
通讯作者：
Haijiao Yan;Jianmin Yun;Duiyuan Ai;Wenwei Zhang;Jiebing Bai;Juan Guo
中科院分区：
文献类型：
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作者：
Haijiao Yan;Jianmin Yun;Duiyuan Ai;Wenwei Zhang;Jiebing Bai;Juan Guo
公众对食品安全和环境问题的关注以及耐杀菌剂病原菌的增加增强了开发杀菌剂替代方法来控制采后水果腐烂的兴趣。本研究从不新鲜的马铃薯粉丝中分离到一株细菌，经形态特征和16 S rRNA基因序列分析，鉴定为短小芽孢杆菌（Bacillus pumilusHN-10）。此外，还从B. pumilusHN-10采用大孔吸附树脂AB-8、Sephadex G-100层析、反相高效液相色谱等方法分离纯化。经NCBI数据库BLAST检索，P-1和P-2的一级结构分别为PLSSPATLNSR和GGSGGGSSGGSIGGR，分子量分别为1142.28和1149.14 Da。P-1和P-2对粉红单端孢菌具有较强的抑菌活性，最低抑菌浓度均为1 μg/mL。这两种新的抗真菌肽在80 °C以下稳定2 h，但在121 °C下15 min内失去活性。此外，它们对胃蛋白酶、胰蛋白酶和木瓜蛋白酶的蛋白水解作用具有抗性，并且在宽范围的pH（2.0-12.0）内稳定。结果表明，P-1和P-2是一种新型的具有特异性抗真菌活性的阳离子抗菌肽。玫瑰。
Public concern for food safety and environmental issues and the increase in fungicide-resistant pathogen have enhanced the interest in developing alternative methods to fungicides to control postharvest fruit decay. In this study, a bacterial strain isolated from stale potato vermicelli was identified asBacillus pumilusHN-10 based on morphological characteristics and 16S rRNA gene sequence analysis. Furthermore, two novel cationic antifungal peptides named P-1 and P-2 were purified fromB. pumilusHN-10 using macroporous adsorbent resin AB-8, Sephadex G-100 chromatography, and reversed-phase high-performance liquid chromatography. The primary structure of P-1 and P-2, which were proved to be novel antifungal peptides by BLAST search in NCBI database, was PLSSPATLNSR and GGSGGGSSGGSIGGR with a molecular weight of 1142.28 and 1149.14 Da, respectively, as indicated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Both P-1 and P-2 exhibited strong antifungal activity againstTrichothecium roseumwith minimum inhibitory concentrations starting from 1 μg/mL. The two novel antifungal peptides were stable below 80 °C for 2 h, but lost their activity in 15 min at 121 °C. In addition, they were resistant to the proteolytic action of pepsin, trypsin, and papain, and stable within a wide range of pH (2.0–12.0). These results showed that P-1 and P-2 are novel cationic antifungal peptides with specific activity againstT. roseum.