THE EFFECT OF INOCULATION WITH LACTOBACILLUS-PLANTARUM ON FERMENTATION IN LABORATORY SILOS OF HERBAGE LOW IN WATER-SOLUBLE CARBOHYDRATE
THE EFFECT OF INOCULATION WITH LACTOBACILLUS-PLANTARUM ON FERMENTATION IN LABORATORY SILOS OF HERBAGE LOW IN WATER-SOLUBLE CARBOHYDRATE
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DOI：
10.1111/j.1365-2494.1990.tb02195.x
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发表时间：
1990-06-01
影响因子：
2.4
通讯作者：
ARMSTRONG, DG
中科院分区：
文献类型：
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作者：
ROOKE, JA;BORMAN, AJ;ARMSTRONG, DG
试验一采用多年生黑麦草和白三叶混合饲料(176g DM kg~(-1))，不添加饲料或添加植物乳杆菌3次。106(g牧草鲜重)-1或肠道细菌，3倍。106(g牧草鲜重)-1或两种接种剂。筒仓在任何一种温度下孵化。摄氏度或摄氏26度。在试验2中，鸡脚菇(247g DM kg~(-1))不加任何添加剂青贮，添加植物乳杆菌4次。106(g牧草鲜重)-1，肠道细菌，4倍。106(g牧草鲜重)-1或5g(kg牧草)-1硝酸铵。筒仓在18℃下孵化。试验1和试验2青贮料中的氨氮浓度分别为139g N(kg全N)和209g N(kg全N)~(-1)，试验1和试验2中未处理的青贮料均未达到满意的品质，S的pH分别为4.3g和6.4，氨氮浓度分别为139g N和209g N(kg全N)-1。试验2发酵较差，反映青贮牧草的水溶性碳水化合物(WSC)含量较低(试验1和试验2分别为136和53g WSC(Kg DM)-1)。接种植物乳杆菌显著降低了青贮料的pH值、氨氮(仅限试验1)和乙醇浓度，增加了乳酸浓度。在每一次实验中，接种肠杆菌的效果都很小。实验一，孵化温度26℃。在26℃下培养7d后，pH值显著降低，C提高了筒仓的发酵速率。C，而不是18度。C，但这种作用在孵育35d后基本消失。延迟封仓48h后，青贮料的pH值和氨氮含量显著高于灌浆后立即封口的青贮料。在试验2中，添加硝酸铵显著降低了7d开封青贮料中乙醇和醋酸的浓度，35d后添加硝酸铵显著降低了乙醇浓度，提高了氨氮浓度。在青贮料中接种硝酸铵后，青贮料的pH值在7~35d显著提高，氨氮和醋酸的含量也显著增加。
In experiment 1, a mixture of perennial ryegrass and white clover (176 g DM kg-1) was ensiled with no addition, or with the application of Lactobacillus plantarum, 3 .times. 106 (g fresh weight of herbage)-1 or enterobacteria, 3 .times. 106 (g fresh weight of herbage)-1 or both of these inoculants. Silos were incubated at either 18.degree. C or 26.degree. C for 7 d and sealing of half of the silos was delayed for 48 h. In experiment 2, cocksfoot (247 g DM kg-1) was ensiled with no additive, with the application of L. plantarum, 4 .times. 106 (g fresh weight of herbage)-1, enterobacteria, 4 .times. 106 (g fresh weight of herbage)-1 or ammonium nitrate, 5 g (kg herbage)-1. Silos were incubated at 18.degree. C and sealing was delayed for 48 h. In neither experiment were the untreated silages of satisfactory quality after 35 d ensilage, having pH''s of 4.3 and 6.4, and ammonia-N concentrations of 139 and 209 g N (kg total N)-1 in experiments 1 and 2 respectively. The poorer fermentation in experiment 2 reflected the lower water-soluble carbohydrate (WSC) content of the ensiled herbage (136 and 53 g WSC (kg DM)-1 in experiments 1 and 2 respectively). Inoculation with L. plantarum significantly reduced silage pH and the concentrations of ammonia-N (experiment 1 only) and ethanol and increased lactic acid concentrations. Inoculation with enterobacteria had only minor effects in each experiment. In experiment 1, incubation at 26.degree. C increased the rate of fermentation in the silos as pH was significantly lower after 7 d incubation at 26.degree. C than at 18.degree. C, but this effect had largely disappeared after 35 d incubation. When sealing of the silos was delayed for 48 h, the resulting silages had significantly higher pH and ammonia-N contents than silages from silos that had been sealed immediately after filling. In experiment 2, addition of ammonium nitrate significantly reduced the concentrations of ethanol and acetic acid in silages opened after 7 d. After 35 d the concentrations of ethanol were significantly reduced and those of ammonia-N increased by the addition of ammonium nitrate. There were marked increases in silage pH between 7 and 35 d ensilage and in the concentrations of ammonia-N and acetic acid in the silages that had been inoculated with ammonium nitrate at ensilage.