Characterization of SMOC-2, a modular extracellular calcium-binding protein
Characterization of SMOC-2, a modular extracellular calcium-binding protein
复制标题
DOI：
10.1042/bj20030532
复制
发表时间：
2003-08-01
影响因子：
4.1
通讯作者：
Hartmann, U
中科院分区：
文献类型：
--
作者：
Vannahme, C;Gösling, S;Hartmann, U
我们已经分离到新的基因Smoc-2，它编码一个分泌的模块蛋白，含有与BM-40同源的EF-手性钙结合结构域。它还由两个甲状腺球蛋白样结构域、一个卵泡抑素样结构域和一个仅在同源Smoc-1中发现的新结构域组成。对钙结合区序列的系统发育分析表明，Smoc-1和Smoc-2形成了一个独立的组。在BM-40系列中。人类和小鼠的Smoc-2序列由分别位于6号和17号染色体上的13个外显子组成的基因编码。对重组表达蛋白的分析表明，Smoc-2是一种钙依赖构象的糖蛋白。Northern杂交和逆转录聚合酶链式反应的结果表明，该基因在许多组织中广泛表达。
We have isolated the novel gene SMOC-2, which encodes a secreted modular protein containing an EF-hand calcium-binding domain homologous to that in BM-40. It further consists of two thyroglobulin-like domains, a follistatin-like domain and a novel domain found only in the homologous SMOC-1. Phylogenetic analysis of the calcium-binding domain sequences showed that SMOC-1 and -2 form a separate group. within the BM-40 family. The human and mouse SMOC-2 sequences are coded for by genes consisting of 13 exons located on chromosomes 6 and 17, respectively. Analysis of recombinantly expressed protein showed that SMOC-2 is a glycoprotein with a calcium-dependent conformation. Results from Northern blots and reverse transcription PCR revealed a widespread expression in many tissues.