Single-Cell RNA Sequencing Reveals Heterogeneity of Vascular Cells in Early Stage Murine Abdominal Aortic Aneurysm-Brief Report.
Single-Cell RNA Sequencing Reveals Heterogeneity of Vascular Cells in Early Stage Murine Abdominal Aortic Aneurysm-Brief Report.
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单细胞RNA测序揭示早期小鼠腹主动脉瘤血管细胞的异质性
DOI：
10.1161/atvbaha.120.315607
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Liu B
中科院分区：
文献类型：
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作者：
Yang H;Zhou T;Stranz A;DeRoo E;Liu B
腹主动脉瘤（AAA）是一种危及生命的血管疾病，其特征是SMC（平滑肌细胞）耗竭、ECM（细胞外基质）降解和免疫细胞浸润。支配AAA主动脉异质性的细胞和分子特征尚待阐明。我们对小鼠AAA组织进行了单细胞RNA测序。通过血管周围应用CaCl 2在C57 BL/6 J小鼠中诱导AAA。无偏聚类鉴定了8种细胞类型的12个不同群体。比较假手术和AAA组织中每个群体的体积和基因表达。此外，我们的特点是在SMC，成纤维细胞和巨噬细胞群体的转录谱和潜在的功能特征，并揭示了在每种细胞类型的独特的调节子。总之，这些数据提供了对小鼠AAA的复杂性和异质性的高分辨率洞察，并表明主要细胞类型（如SMC，成纤维细胞和巨噬细胞）中的群体可能对AAA发病机制有不同的贡献。
AAA (Abdominal aortic aneurysm) is a life-threatening vascular disease characterized by SMC (smooth muscle cell) depletion, ECM (extracellular matrix) degradation, and infiltration of immune cells. The cellular and molecular profiles that govern the heterogeneity of the AAA aorta are yet to be elucidated. We performed single-cell RNA sequencing on mouse AAA tissues. AAA was induced in C57BL/6J mice by perivascular application of CaCl2. Unbiased clustering identified 12 distinct populations of 8 cell types. Percentages of each population and gene expression were compared between sham and AAA tissue. Furthermore, we characterized the transcriptional profiles and potential functional features of populations in SMCs, fibroblasts, and macrophages, and revealed the unique regulons in each cell type. Together, these data provide high-resolution insight into the complexity and heterogeneity of mouse AAA, and indicate that populations within major cell types such as SMCs, fibroblasts, and macrophages may contribute differently to AAA pathogenesis.