Adhesion of adenosine diphosphate-activated platelets to human brain microvascular endothelial cells under flow in vitro is mediated via GPIIb/IIIa
Adhesion of adenosine diphosphate-activated platelets to human brain microvascular endothelial cells under flow in vitro is mediated via GPIIb/IIIa
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二磷酸腺苷激活血小板在体外流动下与人脑微血管内皮细胞的粘附是通过 GPIIb/IIIa 介导的
DOI：
10.1016/s0304-3940(01)01608-1
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发表时间：
2001
影响因子：
2.5
通讯作者：
T. Abe
中科院分区：
文献类型：
--
作者：
N. Tanahashi;Y. Fukuuchi;M. Tomita;Y. Tomita;Koji Inoue;H. Satoh;T. Abe
采用视频增强对比（VEC）显微镜，我们检测了TAK-029 （GPIIb/IIIa拮抗剂）是否能抑制活化血小板对体外人脑微血管内皮细胞（HBEC）的粘附。在玻璃罩上培养HBECs，置于VEC显微镜观察室。然后，用二磷酸腺苷（ADP） （2 μM）激活血小板，以10 s−1的低剪切速率在HBEC上灌注30分钟，洗净。血小板直接粘附在HBEC上。然而，当含ADP （2 μM）和TAK-029 （GPIIb/IIIa拮抗剂，1 μM）的富血小板血浆灌注于HBEC上30分钟并冲洗后，血小板对HBEC的粘附被抑制。抗gpib α抗体（GUR20-5）不抑制adp活化血小板对HBEC的粘附。上述结果表明，体外流动条件下adp活化血小板与HBEC的粘附是通过GPIIb/IIIa介导的
Employing video-enhanced contrast (VEC) microscopy, we examined whether TAK-029 (GPIIb/IIIa antagonist) inhibits the adhesion of activated platelets to human brain microvascular endothelial cells (HBEC) in vitro. HBECs were cultured on a coverglass and put in the observation chamber of VEC microscopy. Then, activated platelets by adenosine diphosphate (ADP) (2 μM) were perfused over HBEC at a low shear rate of 10 s−1for 30 min and washed out. Platelets adhered directly to HBEC. However, platelet adhesion to HBEC was suppressed when platelet rich plasma with ADP (2 μM) plus TAK-029 (GPIIb/IIIa antagonist; 1 μM) was perfused over HBEC for 30 min and washed out. Anti-GPIbα antibody (GUR20–5) did not inhibit adhesion of ADP-activated platelets to HBEC. The above results showed adhesion of ADP-activated platelets to HBEC under flow in vitro is mediated via GPIIb/IIIa
高剪切速率下糖蛋白 IIb-IIIa 复合物介导的不依赖纤维蛋白原的血小板粘附和内皮下血栓形成。
DOI：
10.1172/jci113871
发表时间：
1989
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Weiss,HJ;Hawiger,J;Ruggeri,ZM;Turitto,VT;Thiagarajan,P;Hoffmann,T
通讯作者：
Hoffmann,T