Fragmentation studies of fulvic acids using collision induced dissociation fourier transform ion cyclotron resonance mass spectrometry.
Fragmentation studies of fulvic acids using collision induced dissociation fourier transform ion cyclotron resonance mass spectrometry.
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DOI：
10.1021/ac802624s
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发表时间：
2009-03
影响因子：
7.4
通讯作者：
M. Witt;J. Fuchser;B. Koch
中科院分区：
文献类型：
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作者：
M. Witt;J. Fuchser;B. Koch
采用负离子模式电喷雾电离傅立叶变换离子回旋共振质谱（ESI FTICR MS），利用单体超高分辨质量峰的非共振碰撞诱导解离（CID），对苏万尼河富里酸（SRFA）标准天然复杂有机物进行了分析。前驱体质量的分子式分配导致每个峰的分子式完全相同。分析相应的碎片光谱并与不同标准物质进行比较，揭示了特定的中性损失和碎片模式，导致结构由高羧基和较少羟基组成。不同假同源序列(CH（2)-序列和CH(4) vs O交换）的碎片质量峰比较表明，这些序列之间存在结构上的差异。CID FTICR MS允许在非常复杂的天然有机物质光谱中分离单个质量峰。随后，碎片化对这种材料的结构有了深入的了解。我们的研究结果表明，复杂腐殖质的结构多样性并不像预期的那么高。
The complex natural organic matter standard Suwannee river fulvic acid (SRFA) was analyzed by negative ion mode electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI FTICR MS) using on-resonance collision induced dissociation (CID) of single ultrahigh resolved mass peaks in the ICR cell. Molecular formula assignment of precursor masses resulted in exactly one molecular formula for each of the peaks. Analyses of the corresponding fragment spectra and comparison to different standard substances revealed specific neutral losses and fragmentation patterns which result in structures consisting of a high degree of carboxyl- and fewer hydroxyl groups. The comparison of fragmented mass peaks within different pseudohomologous series (CH(2)-series, and CH(4) vs O exchange) suggested structurally based differences between these series. CID FTICR MS allowed isolating single mass peaks in a very complex natural organic matter spectrum. Subsequently, fragmentation gave structural insights into this material. Our results suggest that the structural diversity in complex humic substances is not as high as expected.