Reliable detection of p53 aberrations in squamous cell carcinomas of the head and neck requires transcript analysis of the entire coding region
Reliable detection of p53 aberrations in squamous cell carcinomas of the head and neck requires transcript analysis of the entire coding region
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DOI：
10.1002/hed.10128
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发表时间：
2002-09-01
影响因子：
2.9
通讯作者：
Bier, H
中科院分区：
文献类型：
--
作者：
Hauser, U;Balz, V;Bier, H
背景资料。P53抑癌基因的异常是头颈部鳞状细胞癌的常见事件。然而，报道的频率差异很大，异常的p53的预测价值仍然是一个有争议的问题。这些不一致可能是由于方法上的限制造成的。对23个SCCHN建立的细胞系进行了p53基因和基因组DNA的序列分析，并用免疫组织化学和Western blotting进行了蛋白质检测。在23例SCCHN中，21例(91%)存在异常或无053，其中4例(19%)位于通常检测的外显子5~9之外。11例存在第二等位基因，4例肿瘤存在相应的转录本。在21例p53异常肿瘤中，仅有12例(57%)可检测到蛋白表达。根据所采用的方法，相当多的SCCHN可能会被错误地归类为P53状态。(C)2002年威利期刊公司。
Background. Aberrations of the p53 tumor suppressor gene are common events in squamous cell carcinomas of the head and neck (SCCHN). However, reported frequencies range considerably, and the predictive value of aberrant p53 is continuing to be an issue of controversy. These inconsistencies are possibly caused by methodical limitations.Methods. In cell lines established from 23 SCCHN, we performed sequence analysis of p53 mRNA and genomic DNA, as well as protein detection using immunohistochemistry and Western blotting.Results. Of the 23 SCCHN, 21 (91%) tumors have aberrant or no 053, including four aberrations (19%) located outside the usually examined exons 5 to 9. A second allele is present in 11, and the respective transcript in 4 tumors. Expression of protein could be detected in only 12 of the 21 p53 aberrant tumors (57%).Conclusions. Depending on the methodical approach applied, a considerable number of SCCHN may be misclassified regarding their p53 status. (C) 2002 Wiley Periodicals, Inc.