[56] Carbodiimide modification of proteins.
[56] Carbodiimide modification of proteins.
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DOI：
10.1016/s0076-6879(72)25060-1
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发表时间：
1972-01-01
影响因子：
--
通讯作者：
Koshland, D E Jr
中科院分区：
文献类型：
--
作者：
Carraway, K L;Koshland, D E Jr
用于修饰蛋白质中的羧基的碳二亚胺亲核程序可在温和的条件下用于所有羧基的定量测定或用于活性研究中选定残基的修饰。该方法允许在活化型碳二亚胺和亲核剂的性质上有多种变化。副作用确实会发生，但可以纠正。
A carbodiimide-nucleophile procedure for modifying carboxyl groups in proteins can be utilized under mild conditions for the quantitative determination of all carboxyl groups or for the modification of selected residues in activity studies. The method allows numerous variations both in the activating carbodiimide and in the nature of the nucleophile. Side reactions do occur but can be corrected for.