Single-Cell RNA Sequencing Reveals the Immune Cell Profiling in IMQ Induced Psoriasis-Like Model.
Single-Cell RNA Sequencing Reveals the Immune Cell Profiling in IMQ Induced Psoriasis-Like Model.
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DOI：
10.2147/jir.s379349
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发表时间：
2022
影响因子：
4.5
通讯作者：
Liu, Jun
中科院分区：
文献类型：
--
作者：
Jin, Shasha;Wang, Yixin;Qie, Chenxin;Yang, Lu;Wu, Yinhao;Zhang, Tingting;Di, Jianwen;Liu, Jun
银屑病是一种慢性全身性炎症性皮肤病，复发率高。免疫反应在银屑病发病中起着重要作用。然而，参与银屑病小鼠炎症反应的免疫细胞亚群还没有得到充分的研究。本研究揭示了银屑病小鼠的免疫环境特点。我们使用单细胞RNA测序(10倍基因组学)作为一种无偏见的分析策略，系统地研究了咪喹莫特诱导的银屑病小鼠皮肤免疫细胞的异质性。我们在14,439个细胞中确定了10个主要簇及其标记基因。银屑病小鼠巨噬细胞、NK/T细胞、常规树突状细胞(CDCs)和浆细胞样树突状细胞(PDCs)比例升高。巨噬细胞是银屑病小鼠最大的巨噬细胞群，并进一步分为7个亚群，所有巨噬细胞群均增多。银屑病小鼠皮肤差异表达基因中，巨噬细胞的Fcgr4、Saa3和Acp5，NK/T细胞的Acp5、Fcgr4和Ms4a6d，CDCs的Saa3和pDCs的Ifit m1在银屑病小鼠皮损中表达上调。京都基因和基因组百科全书(KEGG)信号通路富集化分析强调氧化磷酸化信号和抗原处理和递呈信号在小鼠银屑病样模型中的作用。我们的研究揭示了常用的ImQ诱导的银屑病样模型的免疫环境特征，为系统了解银屑病小鼠的免疫反应提供了系统的见解，有助于比较小鼠模型和人类银屑病模型的异同。
Psoriasis is a chronic systemic inflammatory skin disease with a high recurrence rate. The immune response plays an important role in psoriasis. However, the subsets of immune cells involved in inflammation in psoriatic mice have not been fully studied. This study showed the immune environment characteristics of psoriasis in mice. We used single-cell RNA sequencing (10× Genomics) as an unbiased analytical strategy to investigate the heterogeneity of skin immune cells in imiquimod-induced psoriasis mice systematically. We identified 10 major clusters and their marker genes among 14,439 cells. The proportions of macrophages, NK/T cells, conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) were increased in psoriatic mice. Macrophages were the largest group and were further divided into 7 subgroups, and all macrophage clusters were increased in psoriatic mice. Differentially expressed genes in control versus psoriatic mice skin lesions showed that Fcgr4, Saa3 and Acp5 in macrophages, Acp5, Fcgr4 and Ms4a6d in NK/T cells, Saa3 in cDCs, and Ifitm1 in pDCs were upregulated in psoriasis mice. Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis emphasized the role of oxidative phosphorylation signals and antigen processing and presentation signals in murine psoriasis-like models. Our study reveals the immune environment characteristics of the commonly used IMQ induced psoriasis-like models and provides a systematic insight into the immune response of mice with psoriasis, which is conducive to comparing the similarities and differences between the mouse model and human psoriasis.