Cell-permeable scavengers of superoxide prevent long-term potentiation in hippocampal area CA1
Cell-permeable scavengers of superoxide prevent long-term potentiation in hippocampal area CA1
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DOI：
10.1152/jn.1998.80.1.452
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发表时间：
1998-07-01
影响因子：
2.5
通讯作者：
Klann, E
中科院分区：
文献类型：
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作者：
Klann, E
海马 CA1 区的长时程增强 (LTP) 通常依赖于 N-甲基-D-天冬氨酸 (NMDA) 受体激活。活性氧 (ROS)，包括超氧化物，是响应包括海马体在内的许多大脑区域的 NMDA 受体激活而产生的。在本研究中，使用两种模拟超氧化物歧化酶 (SOD) 的细胞渗透性锰卟啉化合物来确定是否需要产生超氧化物来诱导大鼠海马切片 CA1 区的 LTP。将海马切片与 Mn(III) 四（4-苯甲酸）卟啉 (MnTBAP) 或 Mn(III) 四（1-甲基-4-吡啶基）卟啉 (MnTMPyP) 一起孵育可防止 LTP 的诱导。用光灭活的 MnTBAP 或光灭活的 MnTMPyP 孵育切片对 LTP 的诱导没有影响。 MnTBAP 和 MnTMPyP 均无法逆转预先建立的 LTP。这些观察结果表明，超氧化物的产生是对 LTP 诱导刺激的反应，并且超氧化物是 LTP 诱导所必需的。
Long-term potentiation (LTP) in hippocampal area CA1 is generally dependent on N-methyl-D-aspartate (NMDA) receptor activation. Reactive oxygen species (ROS), including superoxide, are produced in response to NMDA receptor activation in a number of brain regions, including the hipppocampus. In this study, two cell-permeable manganese porphyrin compounds that mimic superoxide dismutase (SOD) were used to determine whether production of superoxide is required for the induction of LTP in area CA1 of rat hippocampal slices. Incubation of hippocampal slices with either Mn(III) tetrakis (4-benzoic acid) porphprin (MnTBAP) or Mn(III) tetrakis (1-methyl-4-pyridyl) porphyrin (MnTMPyP) prevented the induction of LTP. Incubation of slices with either light-inactivated MnTBAP or light-inactivated MnTMPyP had no effect on induction of LTP. Neither MnTBAP nor MnTMPyP was able to reverse preestablished LTP. These observations suggest that production of superoxide occurs in response to LTP-inducing stimulation and that superoxide is necessary for the induction of LTP.