Differential TCR gene usage between WC1- and WC1+ ruminant γδ T cell subpopulations including those responding to bacterial antigen
Differential TCR gene usage between WC1- and WC1+ ruminant γδ T cell subpopulations including those responding to bacterial antigen
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DOI：
10.1007/s00251-006-0122-5
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发表时间：
2006-08-01
期刊：
影响因子：
3.2
通讯作者：
Baldwin, Cynthia L.
中科院分区：
文献类型：
--
作者：
Blumerman, Seth L.;Herzig, Carolyn T. A.;Baldwin, Cynthia L.
根据 WC1 辅助受体（在 γ δ T 细胞上独特表达的富含清道夫受体的半胱氨酸家族成员）的存在或不存在，反刍动物 γ δ T 细胞被分为亚群。有证据表明 WC1(+) 是炎症性的，而 WC1(-) 是调节性的，而且它们的组织分布也不同。最近，这种范式被进一步完善，因为产生干扰素-γ并增殖为自体抗原、钩端螺旋体抗原或IL-12的细胞主要在带有WC1.1抗原表位的WC1(+)亚群中发现，但不带有WC1.2表位。在这里，使用流式细胞术纯化的细胞和逆转录聚合酶链反应（RT-PCR）比较了这些不同亚群（WC1（-）、WC1.1（+）和WC1.2（+））的T细胞受体基因表达。 WC1（-）γδT细胞具有TRG亚组V和C基因的所有11种可能组合的转录本，而WC1（+）亚群中的转录本仅限于TRGV3-TRGC5和TRGV7-TRGC5。相比之下，所有三个亚群都表达所有四种已知牛 TRDV 基因的转录本。对钩端螺旋体抗原刺激培养物中增殖的 WC1(+) γ δ T 细胞的进一步分析表明，根据其 TCR 基因使用情况，它们并不代表更大的 WC1(+) 群体中的独特亚群。此外，对65个转录本的测序表明，它们的连接区域具有多样性，使用了TRGJ5-1、TRGJ5-2、TRDJ1和TRDJ3，并且CDR3的范围为9至24个氨基酸。 WC1.1(+)、WC1.2(+) 和 WC1(+) 抗原反应细胞受限但共享的 γ δ TCR 基因使用使得 WC1 辅助受体成为激活过程和后续反应中重要的决定元件的可能性。
Ruminant gamma delta T cells are divided into subpopulations based on the presence or absence of WC1 co-receptors (scavenger-receptor-cysteine-rich family members uniquely expressed on gamma delta T cells). Evidence suggests WC1(+) are inflammatory while WC1(-) are regulatory and that they also differ in their tissue distribution. Recently, this paradigm was refined further as cells that produce interferon-gamma and proliferate to autologous antigens, leptospira antigens, or IL-12 were largely found within the WC1(+) subpopulation that bears the WC1.1 antigenic epitope but not that bearing the WC1.2 epitope. Here, the T cell receptor gene expression by these different subpopulations (WC1(-), WC1.1(+), and WC1.2(+)) was compared using flow cytometrically-purified cells and reverse transcriptase-polymerase chain reaction (RT-PCR). The WC1(-) gamma delta T cells had transcripts for all 11 possible combinations of the TRG subgroup V and C genes while those in both WC1(+) subpopulations were restricted to TRGV3-TRGC5 and TRGV7-TRGC5. In contrast, all three subpopulations expressed transcripts from all four known bovine TRDV genes. Further analysis of the WC1(+) gamma delta T cells that proliferated in leptospira antigen-stimulated cultures indicated that they do not represent a unique subpopulation within the larger WC1(+) population based on their TCR gene usage. Moreover, sequencing of 65 transcripts showed that their junctional regions were diverse as TRGJ5-1, TRGJ5-2, TRDJ1, and TRDJ3 were used, and CDR3s ranged from 9 to 24 amino acids. The restricted but shared gamma delta TCR gene usage for WC1.1(+), WC1.2(+), and WC1(+)-antigen-responsive cells leaves open the possibility that the WC1 co-receptor is an important determining element in the activation process and subsequent response.