Differential regulation of communication by retinoic acid in homologous and heterologous junctions between normal and transformed cells.
Differential regulation of communication by retinoic acid in homologous and heterologous junctions between normal and transformed cells.
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DOI：
10.1083/jcb.113.2.371
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发表时间：
1991-04
期刊：
影响因子：
--
通讯作者：
Loewenstein WR
中科院分区：
文献类型：
--
作者：
Mehta PP;Loewenstein WR
视黄酸(10（-9)- 10(-8)M）是一种可能的形态形成因子，可大大增加同类型细胞之间连接的通透性（同源连接），多种正常和转化细胞类型都具有这种反应性（Mehta, p.p.， J.S. Bertram, and W.R. Loewenstein. 1989）。[j] .中国生物医学工程学报，2008(8):593 - 593。在这里，我们报告了正常细胞和转化细胞之间的异源连接的反应方向相反；它们的渗透性被维甲酸（大于或等于10(-9)M）及其苯甲酸衍生物四氢四甲基萘丙烯苯甲酸（大于或等于10(-11)M）降低。两类结点的相反响应是并行的；在正常10T1/2细胞与其甲基胆蒽转化的对应物共培养中，异源连接的通透性低于同源连接的通透性（在类维甲酸应用20小时内），同时同源连接的通透性在两种细胞类型中都有所上升。这种反调节至少需要三度的细胞分化。提出了一个模型，其中的分化驻留在一个三重奏的连接通道蛋白。该模型的原理可广泛应用于组织边界（包括胚胎边界）细胞间通讯的调控。
The permeability of junctions between cells of the same type (homologous junctions) is greatly increased by retinoic acid (10(-9)- 10(-8) M), a probable morphogen, and this responsiveness is shared by a variety of normal and transformed cell types (Mehta, P.P., J.S. Bertram, and W.R. Loewenstein. 1989. J. Cell Biol. 108:1053-1065). Here we report that the heterologous junctions between the normal and transformed cells respond in the opposite direction; their permeability is reduced by retinoic acid (greater than or equal to 10(-9) M) and its benzoic acid derivative tetrahydrotetramethylnaphthalenylpropenylbenzoic acid (greater than or equal to 10(-11) M). The opposite responses of the two classes of junction are shown to be concurrent; in cocultures of normal 10T1/2 cells and their methylcholanthrene-transformed counterparts, the permeability of the heterologous junctions, which is lower than that of the homologous junctions to start with, falls (within 20 h of retinoid application), at the same time that the permeability of the homologous junctions rises in both cell types. Such a counter-regulation requires a minimum of three degrees of cellular differentiation. A model is proposed in which the differentiations reside in a trio of junctional channel protein. The principle of the model may have wide applications in the regulation of intercellular communication at tissue boundaries, including embryonic ones.