A FIXATION METHOD FOR VISUALIZATION OF YEAST ULTRASTRUCTURE IN THE ELECTRON-MICROSCOPE
A FIXATION METHOD FOR VISUALIZATION OF YEAST ULTRASTRUCTURE IN THE ELECTRON-MICROSCOPE
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DOI：
10.1007/bf00588651
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发表时间：
1982-01-01
期刊：
影响因子：
5.5
通讯作者：
VISHNIAC, HS
中科院分区：
文献类型：
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作者：
BAHARAEEN, S;VISHNIAC, HS
将含有戊二醛（3%）、丙烯醛（1.5%）和多聚甲醛（1.5%）的初代固定液用0.05 M的酸碱钠（pH 7.2）缓冲，作用于vishniacii隐球菌细胞2 h。然后用6%的高锰酸钾水溶液在室温下处理细胞1小时。这种方法保留了酵母细胞的大部分精细结构成分，包括细胞壁和细胞膜、核膜、线粒体、内质网、微体、液泡、核仁和核糖体。然而，它会导致荚膜物质的破坏和一些脂质和糖原颗粒的损失。
A primary fixative containing glutaraldehyde (3%), acrolein (1.5%) and paraformaldehyde (1.5%) buffered in 0.05 M sodium cacodylate at pH 7.2 was applied to the cells of Cryptococcus vishniacii for 2 h on ice. The cells were then treated with a 6% aqueous solution of potassium permanganate for 1 h at room temperature. This method preserves most of the yeast cell fine structural components including cell walls and membrane, nuclear membrane, mitochondria, endoplasmic reticula, microbodies, vacuoles, nucleoli and ribosomes. However, it leads to disruption of capsular materials and loss of some of the lipid and glycogen granules.