A microfluidic system enabling continuous characterization of specific membrane capacitance and cytoplasm conductivity of single cells in suspension
A microfluidic system enabling continuous characterization of specific membrane capacitance and cytoplasm conductivity of single cells in suspension
复制标题
微流体系统能够连续表征悬浮中单细胞的比膜电容和细胞质电导率
DOI：
10.1016/j.bios.2012.12.035
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发表时间：
2013-05-15
影响因子：
12.6
通讯作者：
Chen, Jian
中科院分区：
文献类型：
--
作者：
Zhao, Yang;Chen, Deyong;Chen, Jian
本文提出了一种微流控系统，能够连续表征悬浮液中单个细胞的比膜电容（C-特异性膜）和细胞质电导率（西格玛（细胞质））。在这项研究中，细胞被连续抽吸通过收缩通道，同时使用显微镜成像和锁定放大器同时测量两个频率（1 kHz和100 kHz）下的细胞伸长和阻抗曲线。使用IkHz阻抗数据来评估具有缩窄通道壁的细胞密封特性，并且将IOOkHz阻抗数据转化为量化单个细胞的等效膜电容和细胞质电阻，其进一步转化为C特异性膜和西格玛（细胞质）。两种模型细胞系（肾肿瘤细胞系786-O（n = 302）和血管平滑肌细胞系T2（n = 216））用于评价该技术，分别产生3.67 +/- 1.00和4.53 +/- 1.51 μ F/cm（2）的C特异性膜和0.47 +/- 0.09和0.55 +/- 0.14 S/m的σ（细胞质）。与先前报道的只能从数十个细胞中收集C特异性膜和sigma（细胞质）的技术相比，这种新技术具有更高的通量，能够在细胞传代后立即在30分钟内从数百个细胞中收集C特异性膜和sigma（细胞质）。(c)2012爱思唯尔有限公司版权所有。
This paper presents a microfluidic system enabling continuous characterization of specific membrane capacitance (C-specific membrane) and cytoplasm conductivity (sigma(cytoplasm)) of single cells in suspension. In this study, cells were aspirated continuously through a constriction channel while cell elongations and impedance profiles at two frequencies (1 kHz and 100 kHz) were measured simultaneously using microscopy imaging and a lock-in amplifier. 1 kHz impedance data were used to evaluate cellular sealing properties with constriction channel walls and 100 kHz impedance data were translated to quantify equivalent membrane capacitance and cytoplasm resistance of single cells, which were further translated to C-specific membrane and sigma(cytoplasm). Two model cell lines (kidney tumor cell line of 786-O (n = 302) and vascular smooth muscle cell line of T2 (n = 216)) were used to evaluate this technique, producing C-specific membrane of 3.67 +/- 1.00 and 4.53 +/- 1.51 mu F/cm(2) and sigma(cytoplasm) of 0.47 +/- 0.09 and 0.55 +/- 0.14 S/m, respectively. Compared to previously reported techniques which can only collect C-specific membrane and sigma(cytoplasm) from tens of cells, this new technique has a higher throughput, capable of collecting C-specific membrane and sigma(cytoplasm) from hundreds of cells in 30 min immediately after cell passage. (c) 2012 Elsevier B.V. All rights reserved.