Prandiology of Drosophila and the CAFE assay
Prandiology of Drosophila and the CAFE assay
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DOI：
10.1073/pnas.0702726104
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发表时间：
2007-05-15
影响因子：
11.1
通讯作者：
Benzer, Seymour
中科院分区：
文献类型：
--
作者：
Ja, William W.;Carvalho, Gil B.;Benzer, Seymour
由于缺乏合适的方法，在遗传上易处理的无脊椎动物模型系统中对摄食行为的研究一直受到限制。我们介绍了毛细管馈线（CAFE），一种方法，允许精确的，实时测量的摄入量由个别或分组果蝇的规模分钟到天。利用这种技术，我们进行了第一次定量分析的果蝇的进餐行为。我们的研究结果允许解剖喂养离散回合的摄入，定义两个单独的参数，膳食量和频率，可以解耦，因此可能是独立的调节。此外，我们的长期测量表明，苍蝇可以在24小时内摄取多达1.7倍的体重。除了研究食欲外，CAFE还可用于监测口服药物的输送。作为一个例子，我们使用CAFE来测试膳食补充剂与两种化合物，百草枯和乙醇，对食物摄入和偏好的影响。百草枯是一种广泛应用于应激试验的促氧化剂，具有很强的促氧化作用。相比之下，在摄食偏好试验中，乙醇掺杂的食物，而不是乙醇本身，作为引诱剂。
Studies of feeding behavior in genetically tractable invertebrate model systems have been limited by the lack of proper methodology. We introduce the Capillary Feeder (CAFE), a method allowing precise, real-time measurement of ingestion by individual or grouped fruit flies on the scale of minutes to days. Using this technique, we conducted the first quantitative analysis of prandial behavior in Drosophila melanogaster. Our results allow the dissection of feeding into discrete bouts of ingestion, defining two separate parameters, meal volume and frequency, that can be uncoupled and thus are likely to be independently regulated. In addition, our long-term measurements show that flies can ingest as much as 1.7 x their body mass over 24 h. Besides the study of appetite, the CAFE can be used to monitor oral drug delivery. As an illustration, we used the CAFE to test the effects of dietary supplementation with two compounds, paraquat and ethanol, on food ingestion and preference. Paraquat, a prooxidant widely used in stress tests, had a strong anorexigenic effect. In contrast, in a feeding preference assay, ethanol-laced food, but not ethanol by itself, acted as an attractant.