Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importin β
Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importin β
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DOI：
10.1016/j.bbrc.2011.01.108
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发表时间：
2011-02-25
影响因子：
3.1
通讯作者：
Yamashita, Toshihide
中科院分区：
文献类型：
--
作者：
Ohara, Ryo;Hata, Katsuhiko;Yamashita, Toshihide
我们描述了以前未被认识到的大鼠胚胎海马神经元轴突发生的现象，并阐明了其潜在的机制。新的神经突从细胞体中产生并生长。这些神经突呈Tau-1阳性，而损伤的轴突对Tau-1呈阴性免疫反应。通过p50的过度表达抑制dynein-dynactin复合物，轴突发生在这些神经元中被延迟。输入蛋白β在轴突切开术后被局部翻译，与动力蛋白-输入蛋白复合物有关，是轴突发生所必需的。综上所述，这些结果表明，损伤诱导的信号在损伤轴突中的逆行转运在轴突切开术诱导的海马神经元轴突发生中起关键作用。(C) 2011爱思唯尔公司版权所有。
We characterize the previously unrecognized phenomenon of axotomy-induced axonogenesis in rat embryonic hippocampal neurons in vitro and elucidate the underlying mechanism. New neurites arose from cell bodies after axotomy and grew. These neurites were Tau-1-positive, and the injured axons showed negative immunoreactivity for Tau-1. Axonogenesis was delayed in these neurons by inhibiting the dynein-dynactin complex through the overexpression of p50. Importin beta, which was locally translated after axotomy, was associated with the dynein-importin a complex and was required for axonogenesis. Taken together, these results suggest that retrograde transport of injury-induced signals in injured axons play key roles in the axotomy-induced axonogenesis of hippocampal neurons. (C) 2011 Elsevier Inc. All rights reserved.